巴氏新小绥螨溴氰菊酯抗药性品系对常见药剂的交互抗性及其种群参数分析

为了明确巴氏新小绥螨Neoseiulus barkeri溴氰菊酯抗性品系对常用杀虫(螨)剂的交互抗性水平及生物适合度，为巴氏新小绥螨抗药性品系田间应用提供科学依据。本文应用玻片浸渍法测定了巴氏新小绥螨溴氰菊酯抗性品系对12种杀虫(螨)剂的交互抗性，采用种群生命表评价了其生长发育及繁殖情况。结果显示，相对于巴氏新小绥螨敏感品系，溴氰菊酯抗性品系(226.38倍)对供试的其他6种拟除虫菊酯类药剂均存在交互抗性，尤其对氯氟氰菊酯的交互抗性倍数最高，达到2 210.91倍。对噻虫嗪、毒死蜱和乙螨唑的交互抗性分别是116.46、110.71倍和20.43倍，对丁硫克百威、阿维菌素和哒螨灵无交互抗性。对其生物适合度研究发现，溴氰菊酯抗性品系相较于敏感品系，前若螨期和产卵前期显著缩短，产卵期和单雌总产卵量显著增加。上述结果表明，巴氏新小绥螨溴氰菊酯抗性品系对10种常用杀虫(螨)药剂产生了不同水平的交互抗性，且其繁殖率显著增加，推荐在田间推广应用。

Cross-resistance to commonly used pesticides and analysis of population parameters in a deltamethrin-resistant strain of Neoseiulus barkeri

This study aims to clarify the cross-resistance level of a deltamethrin-resistant strain of the Neoseiulus barkeri to commonly used pesticides and its fitness, and provide scientific basis for the field application of the strain in biological pest control. The cross-resistance of the deltamethrin-resistant strain to 12 commonly used pesticides was measured by slide-dip method, and the growth and development parameters and the fecundity were determined by the life-table analysis. The results showed that the deltamethrin-resistant strain (226.38 fold) of N. barkeri had cross-resistance to all the other six tested pyrethroids, especially the level of cross-resistance to cyhalothrin is the highest which reached 2 210.91 fold. The strain also showed cross-resistance to thiamethoxam, chlorpyrifos and etoxazole, which was 116.46, 110.71 fold and 20.43 fold, respectively, compared to the sensitive strain. There was no cross-resistance to carbosulfan, abamectin and pyridaben. The results of its biological fitness test reveal that the protonymph stage, pre-oviposition stage were significantly shortened, but the oviposition period and the total eggs laid by single female increased in the resistant strain. These results indicated that the deltamethrin-resistant strain of N. barkeri showed different levels of cross-resistance to nine of commonly used pesticides and their fecundity significantly increased, therefore, it could be more widely used as a biological control agent.