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| 抗黄矮病小麦新品系的分子标记检测研究 | |
| 引用本文： | 王红梅,王浩瀚,岳维云,张正英,欧巧明,陈玉梁.抗黄矮病小麦新品系的分子标记检测研究[J].植物保护,2012,38(3):86-89. |
| 作者姓名： | 王红梅 王浩瀚 岳维云 张正英 欧巧明 陈玉梁 |
| 作者单位： | 1. 甘肃省农业科学院生物技术研究所,兰州,730070 2. 甘肃省张掖市农业科学研究所,张掖,734000 3. 甘肃省天水市农业科学研究所中梁试验站,天水,741000 |
| 基金项目： | 甘肃省农业生物技术研究与应用开发项目(GNSW-2004-03,GNSW-2011-07) |
| 摘 要： | 本研究对以小麦-中间偃麦草异附加系L1和小麦-中间偃麦草部分双二倍体‘无芒中4’为抗源选育出的抗黄矮病小麦新品系进行分子检测和抗病性鉴定.通过应用RAPD、SSR、SCAR 3种分子标记OPF15、Xgwm37、SC-W37进行分子检测,并采用人工接种和大田自然感病的方式进行抗黄矮病鉴定,筛选到了‘93646’、‘2003-2’等高抗黄矮病的小麦新品系.分子检测抗黄矮病基因与田间抗病鉴定结果基本一致,应用的3种PCR标记都可以检测出抗病材料,但SCAR标记SC-W37特异性强、稳定性好,可在小麦抗黄矮病育种早代选择过程中发挥重要作用. |
| 关 键 词： | 小麦黄矮病 分子标记 检测 抗病鉴定 |
Detection of wheat lines with resistance to Yellow dwarf virus by molecular markers |
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| Wang Hongmei,Wang Haohan,Yue Weiyun,Zhang Zhengying,Ou Qiaoming,Chen Yuliang.Detection of wheat lines with resistance to Yellow dwarf virus by molecular markers[J].Plant Protection,2012,38(3):86-89. | |
| Authors: | Wang Hongmei Wang Haohan Yue Weiyun Zhang Zhengying Ou Qiaoming Chen Yuliang |
| Institution: | 1(1.Institute of Biotechnology,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China; 2.Zhangye Institute of Agricultural Sciences,Gansu 734000,China; 3.Zhongliang Station of Agricultural Sciences,Tianshui Institute of Agriculture,Gansu 741000,China) |
| Abstract: | Some BYDV translocation lines were studied by using wheat-Thinopyrum intermedium the addition line L1 and a partial amphiploid ’Zhong 4 awnless’ as the resistance parents.This translocation lines were used to screen molecular markers and identification of disease resistance.A RAPD marker OPF15,SSR marker Xgwm37 and SCAR marker SC-W37 were developed for the BYDV resistance.Some new strains with excellent BYDV resistance were selected by identification of disease resistance and molecular marker,including ’2003-2’ and ’93646’.The results showed that the BYDV resistant materials could be detected by PCR markers.The SCAR marker SC-W37 had high specificity and good reliability,which might play an important role in early-generation selection in wheat breeding. |
| Keywords: | Barley yellow dwarf virus(BYDV) molecular marker detection identification of disease resistance |
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