黄皮酰胺类生物碱的提取及对7种水果病原真菌的抑菌活性

利用菌丝生长速率法测定黄皮果核甲醇提取物对芒果炭疽病菌(Colletotrichum gloeosporioides Penz)等7种水果病原真菌的抑制活性，结果表明该提取物10 mg/mL浓度下对芒果炭疽病菌和香蕉枯萎病菌（Fusarium oxysporum f.sp. cubense）菌丝生长抑制率分别达到87.36%和84.55%。从黄皮果核甲醇提取物中分离得到生物总碱，测定其对上述两种真菌的EC50值分别为0.78 mg/mL和0.81 mg/mL。生物总碱进一步分离纯化得到新肉桂酰胺类化合物lansiumamide B, 在0.08 mg/mL浓度下，对芒果炭疽病菌和香蕉枯萎病菌的菌丝生长抑制率分别为 83.33%和60.78%。

Inhibitory effects of clausenamide alkaloid on seven fruit pathogenic fungi

By using growth rate methods, the antifungal activity of Clausena lansium methanol extract was assayed against 7 fruit pathogenic fungi. The results showed that the antifungal rates of the extract were 87.36％ and 84.55％ to Colletotrichum gloeosporioides and Fusarium oxysporum f. sp. cubense, respectively, at the concentration of 10 mg/mL. Total clausenamide alkaloids was extracted from methanol extract, and the EC50 of total alkaloids against the two fungi was 0.78 mg/mL and 0.81 mg/mL, respectively. Lansiumamide B was further extracted from total alkaloids, and its antifungal rates to the fungi were 83.33% and 60.78%, respectively, at the concentration of 0.08 mg/mL.