甘肃省陇东南大豆孢囊线虫的发生和分布

为了解甘肃省陇东南地区大豆孢囊线虫病分布和发生程度,采用Z字形取样法,对甘肃省陇东南地区9个县(区)37个乡镇大豆孢囊线虫病的发生和分布进行调查,并根据形态特征和rDNA-ITS序列比对鉴定线虫种类。共采集土样411份,陇东地区大豆孢囊线虫检出率为87.2%,其中镇原、西峰、正宁、华池和环县的检出率最高,达100%,镇原孢囊发生量最大,平均孢囊数为11.4个/100 g土;陇南地区大豆孢囊线虫检出率为63.7%,成县和徽县分别为64.0%、63.0%,平均孢囊数分别为0.9和1.2个/100 g土。经形态学特征观测和rDNA-ITS序列比对,甘肃省陇东南采集到的孢囊线虫均鉴定为大豆孢囊线虫Heterodera glycines。

Occurrence and distribution of Heterodera glycines insoutheast of Gansu province

In order to understand the occurrence and distribution of soybean cyst nematode in southeast of Gansu Province, zigzag sampling method was used to investigate soybean cyst nematodes in 37 townships of 9 counties in southeastern Gansu. The species of nematodes were identified based on the morphological observation and rDNA-ITS sequence analysis. Totally, 411 samples of soil samples were collected. The results showed that the detection rate of soybean cyst nematodes in eastern Gansu was 87.2%, among which the detection rates in Zhenyuan, Xifeng, Zhengning, Huachi and Huan County were the highest, with the rates of 100%, while that in Qingcheng was the lowest by 39.3%. The highest density was found in Zhenyuan with the average cysts number of 11.4 per 100 g soil. The detection rate of soybean cyst nematodes in southern Gansu was 63.7% and those in Chengxian and Huixian were 64.0% and 63.0%, respectively, and average densities of nematodes were 0.9 and 1.2 per 100 g soil, respectively. Morphological observation and rDNA-ITS sequence analysis showed that the cyst nematodes collected in southeastern Gansu were identified as Heterodera glycines.