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基于生理指标早期诊断异丙隆对小麦药害的研究
引用本文:孟丹丹,范洁群,郭水良,马晓英,陈 倩,彭泽川,李 涛.基于生理指标早期诊断异丙隆对小麦药害的研究[J].植物保护,2019,45(5):186-189.
作者姓名:孟丹丹  范洁群  郭水良  马晓英  陈 倩  彭泽川  李 涛
作者单位:1. 上海师范大学生命与环境科学学院, 上海 200234; 2. 上海市农业科学院, 上海 201403; 3. 上海低碳农业工程技术研究中心, 上海 201415; 4. 安顺学院农学院, 安顺 561000
基金项目:国家重点研发计划(2018YFD0200500); 国家科技重大专项(2017ZX07202004-004); 上海市科委国内合作计划(18295810500); 上海市科技兴农推广项目[沪农科推字(2018)第4-14号]; 上海市农业科学院卓越团队建设计划[农科创2017(A-03)]; 上海市种业发展项目[沪农科种字(2017)第1-2]
摘    要:为了早期诊断异丙隆对小麦的药害,采用室内生测的方法测定了不同剂量异丙隆作用下小麦株高、地上部鲜重、叶绿素、丙二醛(MDA)、可溶性糖、脯氨酸(Pro)含量和最大光量子产量(F_v/F_m)。结果表明,除可溶性糖外,叶绿素、MDA、Pro、F_v/F_m均能快速响应异丙隆对小麦的胁迫。当50%异丙隆WP施用剂量≥3 000 g/hm~2时,叶绿素a、b、总叶绿素含量和F_v/F_m明显下降,Pro含量明显上升,与清水对照相比均达显著水平,MDA含量随着施药剂量的增加而逐渐上升,当剂量≥2 250 g/hm~2时,小麦MDA含量显著高于清水对照处理;叶绿素、MDA、Pro、F_v/F_m可作为诊断异丙隆对小麦药害的敏感指标。

关 键 词:异丙隆    小麦    药害    早期诊断    最大光量子产量
收稿时间:2018/9/27 0:00:00
修稿时间:2018/11/16 0:00:00

Early diagnosis of isoproturon phytotoxicity on wheat seedlingsbased on physiological indices
MENG Dandan,FAN Jiequn,GUO Shuiliang,MA Xiaoying,CHEN Qian,PENG Zechuan,LI Tao.Early diagnosis of isoproturon phytotoxicity on wheat seedlingsbased on physiological indices[J].Plant Protection,2019,45(5):186-189.
Authors:MENG Dandan  FAN Jiequn  GUO Shuiliang  MA Xiaoying  CHEN Qian  PENG Zechuan  LI Tao
Institution:1. College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China; 2. Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; 3. Shanghai EngineeringResearch Centre of Low-carbon Agriculture, Shanghai 201415, China; 4. College ofAgriculture, Anshun University, Anshun 561000, China
Abstract:For early diagnosis of isoproturon phytotoxicity on wheat seedlings, the plant height, shoot fresh weight, chlorophyll, malondialdehyde (MDA), soluble sugar, proline (Pro) content and Fv/Fm were measured by dose-response experiments in the glasshouse. The results showed that chlorophyll, MDA, Pro content and Fv/Fm except soluble sugar could rapidly respond to isoproturon stress in wheat seedlings. After being treated with isoproturon 50% WP at 3 000 g/hm2 or more, chlorophyll content and Fv/Fm decreased significantly and Pro content increased significantly. The MDA content gradually increased with the increase of isoproturon dosage, after being treated with isoproturon at 2 250 g/hm2 or more; the MDA content was significantly higher than the control. Chlorophyll, MDA, Pro, Fv/Fm can be used as sensitive indicators for early diagnosis of isoproturon phytotoxicity on wheat seedlings.
Keywords:isoproturon  wheat  phytotoxicity  early diagnosis  maximal photochemical efficiency
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