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| 一种植物病毒检测新方法—纳米上转换荧光技术 | |
| 引用本文： | 张明哲,陈曦,吴志毅,张永江,闻伟刚,林晓佳,陈吴健,吴蓉,刘鹏程,李明福.一种植物病毒检测新方法—纳米上转换荧光技术[J].植物保护,2011,37(3):113-116. |
| 作者姓名： | 张明哲 陈曦 吴志毅 张永江 闻伟刚 林晓佳 陈吴健 吴蓉 刘鹏程 李明福 |
| 作者单位： | 1. 浙江出入境检验检疫局,杭州,310012 2. 中国检验检疫科学研究院,北京,100029 3. 宁波出入境检验检疫局,宁波,315012 4. 杭州出入境检验检疫局,杭州,310012 5. 湖州出入境检验检疫局,湖州,313000 |
| 基金项目： | 浙江省自然科学基金项目，浙江出入境检验检疫局科研项目 |
| 摘 要： | 随着大豆进口量的日益增加，大豆携带病毒传入我国的风险在不断增大，高效、快速地进行植物病毒检测，防止外来有害生物入侵，是目前各口岸植物检疫工作的重点。本文介绍了一种植物病毒检测的新方法—纳米上转换荧光技术。该技术是一种利用磁性纳米颗粒(magnetic nanoparticles, MNP)进行病毒分离、富集和定位；同时引入上转换荧光(up converting phosphor, UCP)材料作为标记物的免疫检测方法，具有高度的抗干扰性、多元性、稳定性和安全性等特点。 |
| 关 键 词： | 植物病毒 磁性纳米颗粒 上转换荧光 纳米上转换荧光技术 |
Nano up converting phosphor technology, a new method for detection of plant viruses |
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| Zhang Mingzhe,Chen Xi,Wu Zhiyi,Zhang Yongjiang,Wen Weigang,Lin Xiaoji.Nano up converting phosphor technology, a new method for detection of plant viruses[J].Plant Protection,2011,37(3):113-116. | |
| Authors: | Zhang Mingzhe Chen Xi Wu Zhiyi Zhang Yongjiang Wen Weigang Lin Xiaoji |
| Institution: | Zhang Mingzhe1,Chen Xi1,Wu Zhiyi1,Zhang Yongjiang2,Wen Weigang3,Lin Xiaojia1Chen Wujian1,Wu Rong4,Liu Pengcheng5,Li Mingfu2(1.Zhejiang Entry-Exit Inspection and Quarantine Bureau,Hangzhou 310012,China,2.Chinese Academy of Inspection and Quarantine,Beijing 100029,3.Ningbo Entry-Exit Inspection and Quarantine Bureau,Ningbo 315012,4.Hangzhou Entry-Exit Inspection and Quarantine Bureau,5.Huzhou Entry-Exit Inspection and Quarantine Bureau,Huzhou 313000,China) |
| Abstract: | With the increase of soybean imports,the risk of plant viruses into China has kept increasing.To prevent the invasion of alien species,the efficient and rapid detection method is the focus of plant quarantine.Nano up-converting phosphor technology is based on magnetic nanoparticles(MNP) and up-converting phosphor(UCP) materials.MNP can be used for virus isolation,concentration and positioning;on the other hand,as the marker of immune detection,UCP has the excellent features,such as interference immunity,div... |
| Keywords: | plant virus magnetic nanoparticle up-converting phosphor nano up-converting phosphor technology |
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