发光杆菌NJ菌株的抑菌活性研究

测定了发光杆菌NJ菌株对16种植物病原真菌的抑菌活性,结果表明该细菌具有较广的抑菌谱,其中对甘蓝黑斑病菌、玉米大斑病菌、番茄猝倒病菌、玉米小斑病菌等植物病原真菌具有较强抑制作用。对该菌的发酵条件研究表明,该菌在种龄为24 h、接种量在4%～6%条件下,在TSY培养液中72 h即可获得较高的抑菌活性。而装瓶量为10～50 mL/100 mL锥形瓶,转速为120～200 r/min对菌的活性影响不大。不同温度处理发酵液,其抑菌活性发生变化,其中经60℃处理10 min后,抑菌活性降低约50%,说明发酵液中的抑菌活性成分可能有多种。

A study on the antifungal activity of Photorhabdus sp. NJ

The antifungal activity of Photorhabdus sp. NJ to 16 kinds of plant pathogenic fungus were determined. The results showed that the bacteria showed a wide spectrum in antibiotic activity, especially to Alternaria brassicicola, Exserohilum turcium, Cercospora sojina, Bipolaris maydis and so on. The optimum medium and culture conditions for the NJ strain were studied, which showed that when the stock was cultured for 24 hours, with an inoculation quantity of 4%-6% and 72 hours of fermentation time, the yield of antibiotics could be raised. The antifungal activity changed when the fermentation broth were treated under different temperatures. When treated for 10 minutes under 60 ℃, its inhibiting effect reduced by a half, which indicated that there may be more than one antibiotic substances.