217份一粒系小麦苗期抗条锈病鉴定及抗病基因检测

小麦条锈病是影响小麦生产的重要病害之一。发掘新的抗病基因, 培育新的抗病品种是防治小麦条锈病最经济有效的措施。一粒系小麦作为普通小麦的二级基因源, 蕴含了丰富的抗病资源。为了从一粒系小麦中发掘新抗病基因, 促进其利用, 本文对217份一粒系小麦材料进行了苗期抗条锈病鉴定和A基因组的部分抗条锈病基因检测。结果表明, 共有55份材料在苗期对CYR32表现抗病, 占25.35%;抗病基因分子标记检测显示, 携带Yr1、Yr34/Yr48、Yr69、Yr76抗性基因的材料分别有26、22、16、34份。共有4份表现高抗或免疫的材料同时携带2个抗性基因:2份材料携带Yr69和Yr34, 2份携带Yr1和Yr34。所有供试材料中均未检测到Yr17。此外, 有23份抗病材料未检测到以上抗病基因, 其中7份表现高抗及免疫, 可能携带其他已知或新的抗条锈病基因。本研究为一粒系小麦的利用提供了参考信息。

Identification of stripe rust resistance in the seedling and detection of resistance genes in 217 einkorn wheats

Wheat stripe rust is one of the important diseases affecting wheat production. Exploring new disease-resistant genes and cultivating new disease-resistant varieties are green and effective measures. As the secondary genepool of common wheat (Triticum aestivum L.), einkorn wheat also contains rich disease-resistance resources. In order to discover new disease-resistant genes and promote the utilization of einkorn wheat, the resistance of 217 accessions to stripe rust were identified at the seedling stage and disease-resistant genes on A genome were detected using molecular markers. The results showed that 55 materials exhibited resistance to CYR32 at the seedling stage, accounting for 25.35%, and there were 26, 22, 16 and 34 materials carrying Yr1, Yr34/Yr48, Yr69 and Yr76, respectively. A total of four materials with immune or high resistance carried two resistance genes simultaneously: two materials carried Yr69 and Yr34, and two materials carried Yr1 and Yr34. The gene Yr17 was not detected in the test materials. In addition, 23 materials were not detected for any of the five resistance genes, seven of which showed high resistance or immunity, and might carry other known or new stripe rust resistance genes. This study provides reference information for the utilization of einkorn wheat.