海南橡胶树棒孢霉落叶病菌毒素蛋白基因检测与致病性分析

Cassiicolin毒素蛋白是橡胶树棒孢霉落叶病菌的主要致病因子。本研究对分离自海南省5个市县的橡胶树棒孢霉落叶病菌进行了鉴定,形态学鉴定结合分子生物学鉴定结果表明,病原菌为多主棒孢。利用Cassiicolin毒素不同亚型编码基因特异性引物和菌饼活体接种法分别鉴定了分离菌株的毒素蛋白类型和致病性,结果显示,分离的35个菌株都含有Cas5基因,未发现含其他类型的毒素基因,表明海南橡胶树棒孢霉落叶病菌为含Cas5毒素蛋白优势群体;所有菌株对橡胶树品种‘RRIM600’都能够致病,但是在不同抗性品种上致病性存在一定差异。

Isolation, identification of the cassiicolin and pathogenicity analysis of Corynespora leaf fall disease of Hevea brasiliensis in Hainan

Cassiicolin is the main pathogenic factor of Corynespora cassiicola. In this study, pathogens were isolated from Hevea brasiliensis samples infected with Corynespora leaf fall disease collected from five cities and counties of Hainan province, and were identified as C.cassiicola by morphological and molecular methods. The cassiicolin subtypes and pathogenicity of isolates were analyzed by using specific primers of cassiicolin and inoculation with fungus cake in vivo. The results showed that 35 isolates of C.cassiicola contained Cas5 protein, and no other types of proteins were found. It indicated that Cas5 was the dominant population in Hainan. All isolates of C.cassiicola were found to be pathogenic in ‘RRIM600’ rubber species, but they also showed different pathogenicity in different rubber varieties with resistance to C.cassiicola.