海洋多黏类芽胞杆菌可湿性粉剂的研制

以海洋多黏类芽胞杆菌Paenibacillus polymyxa为研究对象,通过单因素室内试验和田间试验,确定了可湿性粉剂配方及其对甜瓜枯萎病的田间防效。结果表明,可湿性粉剂最佳配方为:以皂土为载体的菌粉70%,稳定剂海藻酸钠14%,湿润剂蔗糖脂肪酸酯8%,分散剂聚乙烯醇8%。制得的可湿性粉剂活菌数为1.23×10^10cfu/g,含水量6%,润湿时间48 s,悬浮率75%,杂菌率0。采用7种不同方法防治甜瓜枯萎病,结果表明,可湿性粉剂对甜瓜枯萎病的综合防治效果高达84.9%。该制剂对甜瓜枯萎病具有良好防效,可作为防治甜瓜枯萎病的药剂在生产上推广应用。

Preparation of Paenibacillus polymyxa wettable powder

Paenibacillus polymyxa was used to study the composition of wettable powder and its field control effect on melon wilt by single factor test in laboratory and field experiments. The optimum formula of the wettable powder was: 70% bacterial powder with bentonite as carrier, 14% sodium alginate as stabilizer, 8% sucrose fatty acid ester as wetting agent and 8% polyvinyl alcohol as dispersant. The number of living bacteria in wettable powder was 1.23×1010 cfu/g, water content 6%, suspension rate 75%, wetting time 48 s and heterogeneous bacteria rate 0%. Seven different methods were used to prevent melon wilt and the results showed that the comprehensive control effect on melon wilt was up to 84.9% by wettable powder. The preparation had a good control effect against melon wilt, and can be popularized and applied as a medicament for controlling melon wilt.