葡萄卷叶伴随病毒1号和3号宁夏分离物部分基因序列分析

为明确葡萄卷叶伴随病毒(GLRaVs)在宁夏贺兰山东麓酿酒葡萄上的侵染状况,采用RT-PCR技术对40份酿酒葡萄样品中的GLRaV-1~GLRaV-5进行了外壳蛋白(CP)、复制酶(RdRp)和热激蛋白(HSP70)基因序列的克隆和分析。检测结果表明,在所检测的5种病毒中,除GLRaV-2和GLRaV-4未检测到外,GLRaV-1和GLRaV-3的检出率最高,分别为20.0%和32.5%,GLRaV-5的检出率仅为5.0%;有6个样品存在GLRaV-1和GLRaV-3两种病毒复合侵染。序列分析表明,GLRaV-1宁夏分离物的部分CP基因序列长度为232nt,其两种分离物间的核苷酸序列同源率为90%,与已报道的国内外其他分离物CP基因序列相比,其同源率为90%~99%;GLRaV-3宁夏分离物的CP基因序列长度为942nt,其两种分离物间的核苷酸序列同源率为40%,与已报道的国内外其他分离物CP基因序列相比,其同源率为40%~99%;GLRaV-3宁夏分离物的RdRp基因序列长度为683nt,其各分离物间的核苷酸序列同源率为90%以上,与已报道的国内外其他分离物RdRp基因序列相比,其同源率为90%~99%;GLRaV-3宁夏分离物的HSP70基因序列长度为546nt,其两种分离物间的核苷酸序列同源率为96%,与已报道的国内外其他分离物HSP70基因序列相比,其同源率为96%~99%。

Sequence analysis of the genes of Grapevine leafroll-associated viruses 1 and 3 from Ningxia isolates

To understand the infection status of Grapevine leaf roll-associated virus in wine grapes grown in Helan Mountain East Region of Ningxia, RT-PCR was used to clone the coat protein (CP), replication enzyme (RdRp) and heat shock protein (HSP70) gene sequences of Grapevine leaf roll-associated virus 1-5 in 40 wine grape samples and gene sequence analysis was conducted. The results showed that the detection rate of GLRaV-1 and GLRaV-3 in all samples was 20.0% and 32.5%, respectively, but the detection rate of GLRaV-5 was only 5.0%; in addition, GLRaV-2 and GLRaV-4 were not detected in the samples, GLRaV-1 and GLRaV-3 showed compound infection in six samples. The sequence analysis showed that each partial sequence of GLRaV-1-CP gene of the two isolates was 232 nt long and their nucleotide sequence homology was 90%, and their nucleotide sequence homology with those of previously reported isolates was 90%-99%. Each of the complete sequence of GLRaV-3-CP gene of the two isolates was 942 nt long, sharing a nucleotide sequence homology of 40%. Their nucleotide sequence homology with those of previously reported isolates was 40%-99%. Each of the complete sequence of GLRaV-3-RdRp gene of the three isolates was 683 nt long, sharing a nucleotide sequence homology of 90%, and their nucleotide sequence homology with those of previously reported isolates was 90%-99%. Each of the complete sequence of GLRaV-3-HSP70 gene of the two isolates was 546 nt long, sharing a nucleotide sequence homology of 96%, and their nucleotide sequence homology with those of previously reported isolates was 96%-99%.