紫外胁迫对棉铃虫成虫保幼激素的影响

采用高效液相色谱法,测定了紫外(Ultraviolet,UV)胁迫对棉铃虫体内保幼激素JHI、JHII和JHIII的影响。结果表明:棉铃虫体内JHI、JHII和JHIII的滴度均随着日龄的变化发生了显著的改变,与1日龄相比,CK、1h/d、5h/d组在2、3、4日龄时的JHI、JHII和JHIII滴度均升高,其中1h/d和5h/d组的JHI、JHII和JHIII滴度在2日龄时达到最高值,且达到显著水平,然后均随着日龄的增大逐渐下降,9h/d组JHI、JHII和JHIII滴度则随着日龄的增大显著降低;棉铃虫体内JHI、JHII和JHIII的滴度随着UV照射时间的延长均发生了显著的变化,当照射时间为5h/d和9h/d时,2～5日龄棉铃虫体内的JHI、JHII和JHIII滴度显著低于对照水平,不同日龄虫体内的JHI、JHII和JHIII滴度均在9h/d时达到最低;此外,日龄与UV照射的交互作用均达到显著水平。

Effects of ultraviolet light stress on juvenile hormone in Helicoverpa armigera adults

The effects of ultraviolet (UV) light stress on juvenile hormone in Helicoverpa armigera adults were studied by HPLC. The results showed that JHI, JHII and JHIII titres in adults changed significantly with the age. Compared with adults at 1 day of age, JHI, JHII and JHIII titres increased in adults at 2 to 4 days of age in the groups of control, 1 h/day and 5 h/day treatments. And the titres reached maximum in adults at 2 days of age with significant differences after exposure for 1 and 5 h/day, and then decreased gradually with the age. When adults were exposed to UV for 9 h/day, JHI, JHII and JHIII titres decreased significantly with age. JHI, JHII and JHIII titres changed significantly with increasing exposure time. Compared to the control, exposure to UV light for 5 h/day and 9 h/day resulted in a significant decline in JHI, JHII and JHIII titres in adults at 2 to 5 days of age. JHI, JHII and JHIII titres in adults reached the minimum after exposure for 9 h/day. The results also showed that the interaction between the age and the exposure time was significant.