Biological control of <Emphasis Type="Italic">Botrytis cinerea</Emphasis> by selected grapevine-associated bacteria and stimulation of chitinase and β-1,3 glucanase activities under field conditions |
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Authors: | Maryline Magnin-Robert Patricia Trotel-Aziz Daniel Quantinet Sylvie Biagianti Aziz Aziz |
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Institution: | 1.Laboratoire d’Eco-Toxicologie, URVVC—EA 2069,Université de Reims,Reims cedex 2,France;2.Laboratoire de Plantes, Pesticides et Développement Durable, URVVC—EA 2069,Université de Reims,Reims cedex 2,France;3.GDV de la Marne,Institut Technique de Champagne,Epernay cedex,France |
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Abstract: | In this study, the biocontrol ability of seven grapevine-associated bacteria, previously reported as efficient against Botrytis cinerea under in vitro conditions, was evaluated in two vineyard orchards with the susceptible cv. Chardonnay during four consecutive
years (2002–2005). It was shown that the severity of disease on grapevine leaves and berries was reduced to different levels,
depending on the bacterial strain and inoculation method. Drenching the plant soil with these bacteria revealed a systemic
resistance to B. cinerea, even without renewal of treatment. Accordingly, this resistance was associated with a stimulation of some plant defense
responses such as chitinase and β-1,3-glucanase activities in both leaves and berries. In leaves, chitinase activity increased
before veraison (end-July) while β-1,3-glucanase reached its maximum activity at ripening (September). Reverse patterns were
observed in berries, with β-1,3-glucanase peaking at full veraison (end-August) and chitinase at a later development stage.
Highest activities were observed with Acinetobacter lwoffii PTA-113 and Pseudomonas fluorescens PTA-CT2 in leaves, and with A. lwoffii PTA-113 and Pantoea agglomerans PTA-AF1 in berries. These results have demonstrated an induced protection of grapevine against B. cinerea by selected bacteria under field conditions, and suggest that induced resistance could be related to a stimulation of plant
defense reactions in a successive manner. |
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Keywords: | Biocontrol Grey mould Induced resistance Rhizobacteria Vitis vinifera |
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