Phylogenetic relationship and genetic diversity of <Emphasis Type="Italic">Agrobacterium</Emphasis> spp. isolated in Poland based on <Emphasis Type="Italic">gyrB</Emphasis> gene sequence analysis and RAPD |
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Authors: | Joanna Pu?awska Monika Ka?u?na |
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Institution: | (1) Research Institute of Horticulture, Pomology Division, ul. Pomologiczna 18, 96-100 Skierniewice, Poland |
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Abstract: | The genetic diversity of 47 strains of Agrobacterium originating from different host plants and geographical locations in Poland, together with 12 strains from other countries
was investigated. It was analyzed using RFLP of DNA fragment amplified with primers UP-1 and UP-2r flanking part of gyrB and parE genes, gyrB sequencing and randomly amplified polymorphic DNA (RAPD) technique. On the basis of obtained results, we found the majority
of agrobacteria isolated in Poland belong to biovar 2. However, among others, three strains distinct from type strains of
all the known Agrobacterium species, were discovered. All three methods showed no correlation between genetic diversity and geographical origin or the
host plant of all studied strains but they revealed high diversity of the tested agrobacteria. The highest diversity was observed
within strains of biovar 1, whereas those of biovar 2 were found to be the more homogenous group. The topology of the constructed
gyrB tree corresponds to topologies of 16S and 23S rDNA trees obtained in this and other studies, but the gyrB tree had deeper branching. In the case of RAPD, it was possible to find a unique DNA fingerprint for almost each strain
tested. The gyrB gene appeared to be a good phylogenetic marker with high discrimination power allowing better differentiation between species
and strains, whereas the RAPD technique can serve as a tool for single strain typing. |
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