<Emphasis Type="Italic">Agrobacterium</Emphasis>-mediated transformation as a useful tool for the molecular genetic study of the phytopathogen <Emphasis Type="Italic">Curvularia lunata</Emphasis> |
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| Authors: | Tong Liu Lixing Liu Xue Jiang Jumei Hou Kehe Fu Feihong Zhou Jie Chen |
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| Institution: | (1) Department of Resources and Environmental Sciences, School of Agriculture and Biology, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai, 200240, People’s Republic of China;(2) Department of Biology and Chemical Engineering, Jindezhen Comprehensive College, Jiangxi, 333000, People’s Republic of China; |
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| Abstract: | In order to explore the molecular mechanisms of virulence and genetic variance of Curvularia lunata in maize, an ATMT (Agrobacterium tumefaciens-mediated transformation) system was established in order to create a wide range of insertional transformants of C. lunata. Our results showed that the germinating conidia were the ideal starting material for transformation. Based on our optimised
transformation condition, the transformation efficiency of C. lunata with T-DNA was improved greatly, and the average transformation frequency was as high as 84 ± 5 transformants per 1 × 106 germlings. Southern blotting results of 39 randomly-selected transformants showed a unique hybridisation pattern and predominant
single-copy insertions. An ATMT library containing approximate 3000 transformants was generated, and four types of transformants
were screened in terms of growth rate, sporulation, mycelial pigmentation, and toxin production in vitro. This library will be used to identify genes involved in the virulence of the pathogen. |
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