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Development of a co-dominant SCAR marker linked to the Ph-3 gene for Phytophthora infestans resistance in tomato (Solanum lycopersicum) |
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| Authors: | Hai Thi Hong Truong Hung Ngoc Tran Hak Soon Choi Pue Hee Park Hye Eun Lee |
| Institution: | 1. Vegetable Research Division, Department of Horticultural Crop, National Institute of Horticultural & Herbal Science (NIHHS), Rural Development Administration (RDA), 540-41 Tap-Dong, Suwon, Korea, 441-440 2. Department of Horticulture Science, Agronomy Faculty, Hue University of Agriculture and Forestry (HUAF), 102 Phung Hung, Hue, Vietnam 3. Department of Biotechnology, Fruit and Vegetable Research Institute (FAVRI), Trau qui, Gia lam, Hanoi, Vietnam |
| Abstract: | Random amplified polymorphism DNA (RAPD) and bulk segregant analysis (BSA) approaches were used to characterize the molecular marker linked to the Phytophthora infestans resistance gene Ph-3 in tomato. A total of 800 RAPD primers were screened. One RAPD marker UBC#602 was identified to be tightly linked to the Ph-3 gene. The marker was successfully converted into a co-dominant sequence characterized amplified region (SCAR) marker. The SCAR marker SCU602 was used to analyze 96 F2 progenies and fitted the expected 1:2:1 Mendelian segregation ratio. Forty one tomato inbred lines were screened using the SCAR marker in comparison with a reference marker linked to the Ph-3 gene and both markers gave the same results. SCU602 was further validated for association to resistance and its potential in MAS in 72 tomato lines and cultivars. The marker identified three genotypes harbouring the resistance allele. This SCAR marker can be used in breeding programs for the selection of the Ph-3 gene for Phytophthora infestans resistance. |
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