Overexpression of StRbohA in Arabidopsis thaliana enhances defence responses against Verticillium dahliae |
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| Institution: | 1. Department of Plant Science, 222 Agriculture Building, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada;2. Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh;3. Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center, Giza, Egypt;4. Department of Plant Pathology, Faculty of Agriculture, Alexandria University, Alexandria, El-Shatby, 21545, Egypt;5. Department of Biology, Faculty of Science, King Khalid University, Abha, 61413, Saudi Arabia;1. Institute of Medical Microbiology, Department of Medicine, University of Zürich, Gloriastrasse 30/32, 8006 Zürich, Switzerland;2. Max von Pettenkofer Institute, Ludwig-Maximilians University Munich, Pettenkoferstrasse 9a, 80336 Munich, Germany;1. Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843, USA;2. Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843, USA;3. These authors contributed equally to this work;1. College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, China;2. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, China;1. Research & Development Center of Biorational Pesticide, Northwest A&F University, Yangling, 712100, Shaanxi, China;2. Engineering and Research Center of Biological Pesticide of Shaanxi Province, Yangling, 712100, Shaanxi, China |
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| Abstract: | Plant NADPH oxidases are key regulators of plant–microbe interactions and reactive oxygen species (ROS) are essential to plant defences against pathogens. A significant part in the role played by ROS has been ascribed to plant respiratory burst oxidase homologs (RBOHs). In potato (Solanum tuberosum), where RBOHs were previously shown to be involved in wound-induced oxidative burst, we assessed their expression after inoculation with Verticillium dahliae Kleb. and showed that StRbohA was the only homolog to be differentially induced in potato in response to inoculation. In order to investigate the potential role of this gene in plant protection against wilt diseases, we used Agrobacterium-mediated transformation of Arabidopsis to assess the effects of its overexpression on plant responses to V. dahliae. After inoculation with this pathogen, the transformed Arabidopsis line overexpressing StRbohA showed lower disease severity (percent damaged leaf area and vascular discoloration) as compared to the wild type. It also had higher ROS production and more cell death caused by hydrogen peroxide (H2O2), compared to the wild type. Suberization of root cells was also more pronounced in the line overexpressing StRbohA, and supports a possible role for StRBOHA in plant resistance to V. dahliae. Together, these findings indicate that overexpressed StRbohA in Arabidopsis enhances the ROS-mediated defence mechanisms against V. dahliae and can be a potential tool to improve plant resistance to this and other soilborne pathogens that cause wilts in economically important crops. |
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| Keywords: | StrbohA Defense ROS NADPH oxidase |
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