Sequence polymorphism of nematode effectors highlights molecular differences among the subspecies of the tobacco cyst nematode complex |
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| Institution: | 1. Invasive Plant Research Laboratory, USDA-ARS, 3225 College Avenue, Ft. Lauderdale, FL 33314, United States;2. Department of Biology, University of Wisconsin, Madison, WI 53706, United States;3. Department of Biology, Texas Christian University, Fort Worth, TX 76129, United States;1. Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea;2. Lifetree Biotech Co., Ltd, Suwon, Gyeonggi-do 441-350, Republic of Korea;3. School of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea;1. College of Pharmacy, Inje University, 607 Obang-dong, Gimhae, Gyeongnam 621-749, Republic of Korea;2. National Creative Research Initiatives (NCRI) Center for Isogeometric Optimal Design, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-744, Republic of Korea;1. Center for Ecological Research, Kyoto University, Otsu, Japan;2. Department of Ecology, Environment and Plant Sciences, Stockholm University, Stockholm, Sweden;1. ICAR-Indian Institute of Millets Research, Hyderabad, 500030, India;2. ICAR-Indian Institute of Maize Research, Ludhuana, 141004, India;3. Center on Rabi Sorghum, Solapur, 413006, India;4. University of Agricultural Sciences, Dharwad, 580005, India;5. Vasantrao Naik Marathwada Agricultural University, Parbhani, 431401, India;6. University of Agricultural Sciences, Raichur, RARS, Kalaburagi, 585102, India;1. Department of Ecology, University of Debrecen, Debrecen, Egyetem square 1, H-4032 Hungary;2. Hungarian Department of Biology and Ecology, Babeş-Bolyai University, Str. Clinicilor nr. 5–7, 400006 Cluj-Napoca, Romania;3. MTA-DE Biodiversity and Ecosystem Services Research Group, Debrecen, Egyetem square 1, H-4032 Hungary |
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| Abstract: | Globodera tabacum is a little-known species complex of specialized plant–parasitic nematodes. In this study we investigated for the first time the sequence variability of five effectors: three genes coding for cell wall degrading enzymes (Pel1, Pel2 and ExpB3) and two genes coding for CLE peptides (CLE1 and CLE4) among a set of populations representing the different subspecies described. Substantial variability was observed in the sequence dataset which is consistent with the differential pathogenicity of the G. tabacum subspecies on a range of Nicotiana species. Using these sequence data, we were able (1) to show the presence of a novel CLE-like class in cyst nematodes, (2) to support the existence of most probably three and not four subspecies in this species complex, (3) to reveal that the subspecies G. tabacum tabacum presents the highest diversity and (4) to develop a PCR tool that allows a quick and reliable identification of this subspecies among the species complex. |
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| Keywords: | Pectate lyase Expansin Clavata like ESR Species complex Diagnostic |
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