Biological control mechanisms of D-pinitol against powdery mildew in cucumber |
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| Institution: | 1. Plant Health and Natural Products Lab, Key Lab of Urban Agriculture (South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China;2. Institut de Recherche pour le Développement, UMR 186 IRD-Cirad-UM2 Résistance des Plantes aux Bioagresseurs, 911 avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France;1. Center for Forest Research, Université du Québec à Montréal, P.O. Box 8888, Succursale Centre-ville, Montréal, QC, H3C 3P8, Canada;2. Facultad de Ciencias Agrarias, Universidad del Cauca, Calle 5 N° 4-70, Popayán, Colombia;3. Programa de Biología, Facultad de Ciencias Naturales y Matemáticas, Universidad del Rosario, Carrera 24 No. 63C-69, Bogotá, Colombia;4. Institute of Temperate Forest Sciences (ISFORT), University of Quebec in Outaouais (UQO), 58 Rue Principale, Ripon, QC, J0 V 1V0, Canada;1. Centro Nacional de Referencia de Control Biológico, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110 Tecomán, Colima, Mexico;2. Unidad Académica de Ciencias Biológicas, Universidad Autónoma de Zacatecas, Av. Preparatoria S/N, Campus Universitario II, Col. Agronómica, C.P. 98066 Zacatecas, Mexico;3. Departamento de Biología Celular y Molecular, Universidad de Guadalajara, Centro Universitario de Ciencias Biológicas y Agropecuarias, Camino Ing. Ramón Padilla Sánchez 2100, Nextipac, C.P. 45510 Zapopan, Jalisco, Mexico;1. Department of Medical Biotechnology, College of Medicine and Public Health, Flinders University, Bedford Park 5042, South Australia, Australia;2. Soil Biology and Diagnostics, South Australian Research and Development Institute, Urrbrae 5064, South Australia, Australia;1. Department of Biology and Chemistry, Bozhou College, Bozhou 236800, China;2. Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036, China;3. College of Landscape and Art, Jiangxi Agricultural University, Nanchang 330045, China;1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Horticulture, China Agricultural University, Beijing, 100193, China;2. National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China;3. Shandong Provincial Key Laboratory of Cucurbitaceae Vegetable Biological Breeding, Shandong Huasheng Agriculture Co. Ltd, Qingzhou, Shandong, 262500, China;4. College of Plant Science & Technology, Beijing University of Agriculture, Beijing, 102206, China |
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| Abstract: | D-pinitol is an effective agent for controlling powdery mildew (Podosphaera xanthii) in cucumber. In this study, we determined the mechanisms of D-pinitol in controlling powdery mildew in cucumber plants. We compared P. xanthii development on cucumber leaf surface treated with D-pinitol or water (2 mg ml?1) at different time points after inoculation. The germinating conidia, hyphae, and conidiophores of the pathogen were severely damaged by D-pinitol at any time of application tested. The highest level of suppression of fungal development was obtained at 3 days after inoculation. The contents of chlorophyll, total phenolics, flavonoid, and gallic acid and its derivatives (GAD); the activities of phenylalanine ammonialyase (PAL), polyphenoloxidase (PPO), peroxidase (POX), and superoxide dismutase (SOD); and the expression of the genes encoding for PR-1, peroxidase (POX), lipoxygenase (LOX1), chitinase (Chit1) were higher in the cucumber leaves treated with D-pinitol and inoculated than in the leaves either treated with D-pinitol or inoculated with the pathogen. These results suggest that D-pinitol triggers several plant defense responses in cucumber leading to pathogen suppression and resistance to powdery mildew. |
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| Keywords: | Plant defense responses D–pinitol Cucumber |
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