Biochemical defense responses of black pepper (Piper nigrum L.) lines to Phytophthora capsici |
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| Institution: | 1. Indian Institute of Spices Research, Marikunnu P O, Kozhikode 673012, Kerala, India;2. Indian Institute of Horticulture Research, Bangaluru 560089, Karnataka, India;1. Department of Life Sciences, Presidency University, Kolkata, India;2. MMHS, Joynagar, India;3. Department of Biotechnology, Manipur University, Imphal, India;4. Department of Biotechnology, Lovely Faculty of Technology and Sciences, Lovely Professional University, Jalandhar, India;1. School of Agriculture and Food Sciences, John Hines (Bld 62), The University of Queensland, St Lucia, 4072, Australia;2. Queensland Department of Agriculture Fisheries and Forestry, Maroochy Research Station, PO Box 5083, SCMC, Nambour, 4560, Australia;3. Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA;1. Unité de Recherche en Génomique Végétale (URGV), UMR INRA/CNRS/Université d’Evry Val d’Essonne/Saclay Plant Sciences, 2 rue Gaston Crémieux, 91057 Evry, France;2. Center for Desert Agriculture, 4700 King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia;1. Dipartimento Scienze della Vita, Università di Siena, via Mattioli 4, 53100, Siena, Italy;2. Dipartimento Scienze Biologiche, Geologiche e Ambientali, Università di Bologna, via Irnerio 42, 40126, Bologna, Italy;1. Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan;2. Department of Chemistry, University of Karachi, Karachi, Pakistan;3. Colorcon Limited, Crossways, Victory Way, Kent, Dartford, England |
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| Abstract: | A study on biochemical factors involved in black pepper defense response against Phytophthora capsici, was carried out in P. capsici susceptible (Sreekara) and resistant (04-P24, shows root resistance to the pathogen) black pepper lines. Seven important factors – change in membrane conductance, total phenols, orthodihydroxy (OD) phenols, lignin and defense related enzymes (peroxidase, β-1,3 glucanase and β-1,4 glucanase) – were studied under uninoculated and pathogen (P. capsici, isolate 06-04) inoculated condition to know the preformed and induced responses. The pathogen was inoculated (soil inoculation) and plants were observed for changes, at 24 h intervals for 10 days. On 8th day after inoculation symptoms started appearing on Sreekara and increased the severity till 10th day. Both root and stem samples were subjected for biochemical analysis. Of the factors analyzed, it was found that membrane conductance, OD phenol, lignin and peroxidase activity play significant role in root resistance to P. capsici in 04-P24. Light microscopy of the portion of root – where pathogen found attached – was also done. |
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| Keywords: | Defense related enzymes Lignin Membrane conductance Orthodihydroxy phenol Total phenols |
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