禾谷缢管蚜翅型分化与共生菌的关联

本研究旨在探究共生菌与禾谷缢管蚜Rhopalosiphum padi(Linnaeus)翅型分化之间的联系,丰富对昆虫表型可塑性作用机理的认知.利用Illumina MiSeq高通量测序技术对禾谷缢管蚜体内细菌的16S rDNA的V3-V4变异区进行测序分析.从所有样品中鉴定出174个可操作分类单元(OTU),隶属于1...

The Relationship between Symbiotic Bacteria and Wing Dymorphism in Rhopalosiphum padi

The purpose of this study was to explore the relationship between symbiotic bacteria and the wing dimorphism in Rhopalosiphum padi (Linnaeus) and to enrich the understanding of the mechanism of insect phenotypic plasticity. This study used Illumina MiSeq high-throughput sequencing platform to sequence the V3-V4 regions of the bacterial 16S rDNA gene in the alate and apterous morphs of R. padi, respectively. One hundred and seventy-four operational taxonomic units (OTUs) belonging to 10 phyla, 18 classes, 42 orders, 66 families and 98 genera were identified from all samples. At the genus level, Buchnera, as the dominant bacteria, had the highest abundance (83.21%), followed by Staphylococcus (5.25%) and Arthrobacter (2.08%). The diversity of symbionts varied between the two morphs. Apterous morph had more bacterial diversity (174 OTUs belonging to 10 phyla, 18 classes, and 66 families) than alate morphs (151 OTUs belonging to 9 phyla, 14 classes, and56 families). The abundance of various OTUs were significantly different between these two morphs. Among these OTUs, only Buchnera represented a high proportion (95.57%) in alate morphs but were present in low abundance (70.84%) in apterous morphs. The survey showed that there were differences in the diversity and abundance of symbionts between winged and wingless R. padi. These results are helpful to further understand the microorganisms that may be involved in insect wing dimorphism, and contribute to control the dispersal of this pest by reinfection of symbionts or targeting symbiosis-related host genes by RNA interference in future.