| 黄瓜炭疽病拮抗细菌的筛选及其抑制效果 |
| |
| 引用本文： | 谢学文,董瑞利,石延霞,柴阿丽,朱发娣,李宝聚.黄瓜炭疽病拮抗细菌的筛选及其抑制效果[J].中国生物防治学报,2016,32(2):215-220. |
| |
| 作者姓名： | 谢学文 董瑞利 石延霞 柴阿丽 朱发娣 李宝聚 |
| |
| 作者单位： | 中国农业科学院蔬菜花卉研究所, 北京 100081 |
| |
| 基金项目： | 国家公益性行业(农业)科研专项(201203095)，中国农业科学院科技创新工程(CAAS-ASTIP-IVFCAAS)，农业部园艺作物生物学与种质创制实验室 |
| |
| 摘 要： | 从连作多年的黄瓜根际土样中筛选得到1株对黄瓜炭疽病菌Colletotrichum orbiculare具有较强拮抗活性的菌株WF-3。通过菌落形态观察、生理生化特性及16S rDNA序列分析,该菌株鉴定为甲基营养型芽孢杆菌Bacillus methylotrophicus。活体盆栽条件下,该菌株对黄瓜炭疽病的防治效果为66.48%。两年的田间试验表明拮抗菌株WF-3培养物对黄瓜炭疽病具有良好的防治效果,防效分别为68.14%和73.70%。
|
| 关 键 词： | 黄瓜炭疽病菌 拮抗细菌 甲基营养型芽孢杆菌 黄瓜 |
| 收稿时间： | 2015-05-30 |
Screening and Inhibition Effect of Antagonistic Bacteria against Cucumber Anthracnose Caused by Colletotrichum orbiculare |
| |
| XIE Xuewen,DONG Ruili,SHI Yanxia,CHAI Ali,ZHU Fadi,LI Baoju.Screening and Inhibition Effect of Antagonistic Bacteria against Cucumber Anthracnose Caused by Colletotrichum orbiculare[J].Chinese Journal of Biological Control,2016,32(2):215-220. |
| |
| Authors: | XIE Xuewen DONG Ruili SHI Yanxia CHAI Ali ZHU Fadi LI Baoju |
| |
| Institution: | Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
| |
| Abstract: | A bacterial strain WF-3 isolated from rhizosphere soil of cucumber in a continuous cucumber cropping system, exhibited a high antagonistic effect on cucumber anthracnose caused by Colletotrichum orbiculare. This strain was identified as Bacillus methylotrophicus according to its morphological, physiological and biochemical characterizations and the analysis of 16S rDNA gene sequence. In this paper, we have investigated the control effect of WF-3 against cucumber anthracnose via pot and field experiments. Results showed that in the pot experiment the control effect was 66.48%, while in a two-year field test conducted with the cultures of WF-3, the good control effect of 68.14% and 73.70% was obtained in 2013 and 2014, respectively, which proved that WF-3 can effectively control cucumber anthracnose. |
| |
| Keywords: | Colletrichum orbiculare antagonistic bacteria Bacillus methylotrophicus cucumber |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国生物防治学报》浏览原始摘要信息 |
| 点击此处可从《中国生物防治学报》下载免费的PDF全文 |
|
