受黄色镰刀菌侵染的马铃薯薯块的抗氧化酶、细胞壁防御酶及StLTPa1基因的表达特性

黄色镰刀菌Fusarium culmorum为黑龙江省马铃薯干腐病主要致病菌,干腐病可以导致马铃薯在窖藏过程中发生腐烂,影响薯块的商品价值和食用价值,为进一步研究马铃薯干腐病的发生和防治,本研究采用黄色镰刀菌对不同抗性的马铃薯块茎进行侵染,对病原菌侵染过程中薯块的抗氧化酶及细胞壁降解酶变化及病程相关基因的表特性进行了研究。结果表明,当黄色镰刀菌侵染块茎时,块茎中的可溶性蛋白和丙二醛(malondlaldehyde,MDA)含量,超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)活性、几丁质酶(chitinase)和β-1,3-葡聚糖酶(β-1,3-glucanase)活性都呈现不同程度的上升趋势。非特异性脂质转移蛋白基因StLTPa1表达量随着黄色镰刀菌的侵染时间呈波动性,在植物防御反应中发挥一定的作用。

Activities of Antioxidative and Cell Wall Defense Enzymes and Expression of StLTPa1 Gene in Fusarium culmorum-infected Potato Tuber

Fusarium culmorum is the main pathogenic fungus for potato dry rot in Heilongjiang Province. This disease causes corruption of stored potato tubers, affecting their commodity value. In order to understand the occurrence of the disrase, we analyzed physiological and biochemical defenses from the tubers in respect toantioxidative enzymes, cell wall degrading enzyme and expression profiles of disease related genes during F. culmorum infection progress. The results showed that the content of soluble proteins, MDA content, SOD activity, chitinase activity and β-1,3-glucanase activity increased during F.culmorum infection. In addition, the expression level of non-specific lipid transfer protein gene-StLTPa1 showed fluctuation in the entire infection process. These performances may play a role in plant defense responses.