亚洲玉米螟和黏虫幼虫取食Cry1F蛋白后的中肠组织病理变化

为了明确Cry1F杀虫蛋白对亚洲玉米螟和黏虫两种重要玉米害虫的作用机制，本文通过透射电镜观察了亚洲玉米螟和黏虫4龄幼虫取食含Cry1F蛋白人工饲料后的中肠组织病理变化，并与其取食含Cry1Ab蛋白人工饲料后的组织病理变化进行了比较。亚洲玉米螟和黏虫4龄幼虫取食Cry1F蛋白48 h后中肠杯状细胞发生了明显的病变，主要表现为：细胞微绒毛肿胀、脱落；细胞核变形，质膜和核膜界限不清晰；染色质发生固缩、贴近核膜；线粒体拉伸变形数量减少，严重的发生空泡化；内质网肿胀与断裂、数量减少，与取食含Cry1Ab蛋白人工饲料相比，玉米螟和黏虫中肠组织发生的病变程度相似。本研究可为Cry1F作为转基因玉米重要的杀虫蛋白在未来亚洲玉米螟和黏虫防治中更好地发挥作用提供理论依据。

Pathological Changes in Larval Midgut Tissues of Ostrinia furnacalis (Lepidoptera:Crambidae) and Mythimna separata (Lepidoptera:Noctuidae) after Feeding Cry1F Protein

To determine the action mechanism of the insecticidal protein Cry1F, the pathological changes in the midgut of the Asian corn borer, Ostrinia furnacalis (Guené) and Oriental armyworm, Mythimna separata (Walker), fed on an artificial diet containing Cry1F protein, were observed using transmission electron microscope, and the changes induced by Cry1Ab were also investigated. The results showed that the goblet cells changed significantly after Asian corn borer and Oriental armyworm fed on an artificial diet containing Cry1F protein for 48 h, the major symptoms included:microvilli swelled and dropped off, the nucleus deformation, the boundary of karyotheca in cell nucleus blurred and the chromatins underwent pyknosis and was pressed close to the karyotheca, the number of mitochondria tensile deformation and endoplasmic reticulum reduced, the inner cristae of mitochondria indistinct and vacuolated, and the endoplasimic reticulum were swollen, fractured and disordered. The pathological changes of goblet cells in the midgut of Asian corn borer and Oriental armyworm larvae induced by Cry1F were the similar to that by Cry1Ab. This study may provide the theoretical basis for better using Cry1F as important insecticidal protein in transgenic corn in the future.