| 黄瓜白粉病菌拮抗真菌鉴定及生防效果评价 |
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| 引用本文： | 卢幸蓉,陈大为,张树武,刘佳,徐秉良.黄瓜白粉病菌拮抗真菌鉴定及生防效果评价[J].中国生物防治学报,2017(3):427-432. |
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| 作者姓名： | 卢幸蓉 陈大为 张树武 刘佳 徐秉良 |
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| 作者单位： | 甘肃农业大学植物保护学院/甘肃省农作物病虫害生物防治工程实验室,兰州,730070 |
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| 基金项目： | 甘肃省国际科技合作专项(1604WKCA010)，甘肃省农牧厅生物技术专项(GNSW-2013-19)，国家重点研发计划(2016YFD0201112) |
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| 摘 要： | 本文对黄瓜白粉病菌Sphaerotheca fuliginea拮抗真菌进行了筛选和鉴定及防治效果的评价。结果表较高的拮抗活性。菌株JK-13发酵液处理白粉菌孢子48 h后,其对孢子萌发抑制率为89.93%;发酵液处理发病的离体叶片和盆栽幼苗7 d后,其防治效果分别为88.02%和76.34%。利用形态学和分子生物学技术鉴定该菌为腐皮镰刀菌Fusarium solani。
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| 关 键 词： | 黄瓜白粉病 拮抗真菌 鉴定 生物防治 |
Identification of Antagonistic Fungus against Sphaerotheca fuliginea and Evaluation of Its Biocontrol Efficacy |
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| LU Xingrong,CHEN Dawei,ZHANG Shuwu,LIU Jia,XU Bingliang.Identification of Antagonistic Fungus against Sphaerotheca fuliginea and Evaluation of Its Biocontrol Efficacy[J].Chinese Journal of Biological Control,2017(3):427-432. |
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| Authors: | LU Xingrong CHEN Dawei ZHANG Shuwu LIU Jia XU Bingliang |
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| Abstract: | The antagonistic fungus against the pathogen Sphaerothecafuliginea were isolated and assessed in this paper.The resulted showed that 21 strains of antagonistic fungus were isolated from the edge of cucumber tissue infected by S.fuliginea.Among all the isolated strains,the strain JK-13 presented the highest control efficacy in vitro and greenhouse experiments.The inhibitory rate on S.fuliginea spores germination was 83.93％ after treated by strain JK-13 fermentation broth for 48 h.The control efficacy of strain JK-13 fermentation broth on S.fuliginea were 88.02％ and 76.34％ after treated for 7 d by leaf disc and pot experiments,respectively.Based on the morphological characteristic and molecular identification,the strain JK-13 was identified as Fusarium solani. |
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| Keywords: | cucumber powdery mildew antagonistic fungus identification biological control |
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