| 香蕉3种主要病毒的多重PCR检测 |
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| 引用本文： | 李媛媛,吴竹妍,黎园,饶雪琴.香蕉3种主要病毒的多重PCR检测[J].植物检疫,2011(4). |
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| 作者姓名： | 李媛媛 吴竹妍 黎园 饶雪琴 |
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| 作者单位： | 华南农业大学资源环境学院; |
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| 基金项目： | 华南农业大学校长基金(4200-K07324) |
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| 摘 要： | 根据香蕉束顶病毒(Banana bunchy top virus,BBTV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)和香蕉线条病毒(Banana streak virus,BSV)3种病毒核酸保守序列设计相应特异性引物,通过体系优化,建立了可以同时检测香蕉束顶病毒、黄瓜花叶病毒、香蕉线条病毒的多重PCR体系。
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| 关 键 词： | 多重PCR 香蕉束顶病毒 黄瓜花叶病毒 香蕉线条病毒 |
Studies on simultaneous detection of three main banana viruses by multiplex PCR |
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| Li Yuanyuan,Wu Zhuyan,Li Yuan,Rao Xueqin.Studies on simultaneous detection of three main banana viruses by multiplex PCR[J].Plant Quarantine,2011(4). |
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| Authors: | Li Yuanyuan Wu Zhuyan Li Yuan Rao Xueqin |
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| Institution: | Li Yuanyuan,Wu Zhuyan,Li Yuan,Rao Xueqin*(College of Natural Resources and Environment,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | According to the conserved sequences of Banana bunchy top virus(BBTV),Cucumber mosaic virus(CMV) and Banana streak virus(BSV),three specific primer pairs were designed,through optimizing the system,a Multiplex-PCR were established to detect the RNA(CMV) and DNA(BBTV,BSV) simultaneously |
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| Keywords: | Multiplex PCR Banana bunchy top virus(BBTV) Cucumber mosaic virus(CMV) Banana streak virus(BSV) |
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