| 大洋臀纹粉蚧和南洋臀纹粉蚧TaqMan实时荧光PCR检测方法 |
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| 引用本文： | 徐浪,余道坚,焦懿,王银竹,陈志粦.大洋臀纹粉蚧和南洋臀纹粉蚧TaqMan实时荧光PCR检测方法[J].植物检疫,2010,24(2). |
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| 作者姓名： | 徐浪 余道坚 焦懿 王银竹 陈志粦 |
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| 作者单位： | 1. 深圳出入境检验检疫局,广东深圳,518001
2. 中山大学昆虫学研究所有害生物控制与利用国家重点实验室 |
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| 基金项目： | 深圳出入境检验检疫局科研项目 |
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| 摘 要： | 臀纹粉蚧属有多个种类是重要的农业害虫,大洋臀纹粉蚧(Planococcus minor(Maskell))和南洋臀纹粉蚧(Planococcus lilacius Cockerell)是我国有重要检疫意义的有害生物.这两种臀纹粉蚧经常从进口泰国和东南亚水果口岸检疫中截获,但形态学方法很难进行准确鉴定.本研究首次利用mtDNA COI基因设计了两条特异性探针,应用TaqMan实时荧光PCR方法对大洋臀纹粉蚧和南洋臀纹粉蚧进行了快速准确鉴定.
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| 关 键 词： | 大洋臀纹粉蚧 南洋臀纹粉蚧 TaqMan实时荧光PCR MGB探针 |
TaqMan Real-time qualitative PCR for the inspection and identification of Planococcus minor and P. lilacius (Homoptera:Pseudococcidae) |
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| Xu Lang,Yu Daojian,Jiao Yi,Wang Yinzhu,Chen Zhilin.TaqMan Real-time qualitative PCR for the inspection and identification of Planococcus minor and P. lilacius (Homoptera:Pseudococcidae)[J].Plant Quarantine,2010,24(2). |
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| Authors: | Xu Lang Yu Daojian Jiao Yi Wang Yinzhu Chen Zhilin |
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| Institution: | Xu Lang1,Yu Daojian1,Jiao Yi1,Wang Yinzhu2,Chen Zhilin1(1.Shenzhen Entry-Exit Inspection , Quarantine Bureau,Shenzhen518001,China,2.Institute of Entomology , State Key Laboratory for Biocontrol,Sun Yat-Sen University) |
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| Abstract: | Mealybugs in the genus Planococcus encompass several agricultural pests.The mealybug species Planococcus lilacius Cockerell and Planococcus minor(Maskell)(Homoptera:Coccoidea:Pseudococcidae) have special significance to China quarantine.The two mealybugs species are frequently intercepted from the fresh fruits importing from Thailand and the Southeast Asia.The specimens are difficult to identify based on morphological characters.In this study,two sets of specific PCR primers and probes were obtained based o... |
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| Keywords: | Planococcus minor Planococcus lilacius TaqMan Real-time PCR MGB probe |
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