| 油菜茎基溃疡病菌DPO-PCR检测方法的建立 |
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| 引用本文： | 龙阳,袁俊杰,候翠丽,卢乃会,杨卓瑜,马新华,魏霜.油菜茎基溃疡病菌DPO-PCR检测方法的建立[J].植物检疫,2017(4):42-45. |
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| 作者姓名： | 龙阳 袁俊杰 候翠丽 卢乃会 杨卓瑜 马新华 魏霜 |
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| 作者单位： | 1. 湛江出入境检验检疫局检验检疫技术中心 广东湛江 524000;2. 中国检验认证基团广东有限公司湛江分公司;3. 广东出入境检验检疫局检验检疫技术中心 |
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| 基金项目： | 国家质检总局科技项目(2015IK080)，广东出入境检验检疫局科技计划项目(2016GDK32) |
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| 摘 要： | 根据油菜茎基溃疡病菌ITS基因序列,设计特异性DPO引物,建立检测油菜茎基溃疡病菌的DPO-PCR检测方法,并对其特异性、灵敏性进行评价。结果显示与常规PCR检测方法相比,DPO-PCR反应对退火温度不敏感,具有更强的特异性。利用该方法对10批油菜籽样品进行检测,共检出5批阳性样品,检测结果与常规PCR一致,为油菜茎基溃疡病菌的检测提供了新方法。
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| 关 键 词： | 油菜茎基溃疡病菌 DPO-PCR 检测 |
Development of DPO-PCR method for the detection of Leptosphaeria maculans |
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| Long Yang,Yuan Junjie,Hou Cuili,Lu Naihui,Yang Zhuoyu,Ma Xinhua,Wei Shuang.Development of DPO-PCR method for the detection of Leptosphaeria maculans[J].Plant Quarantine,2017(4):42-45. |
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| Authors: | Long Yang Yuan Junjie Hou Cuili Lu Naihui Yang Zhuoyu Ma Xinhua Wei Shuang |
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| Abstract: | A pairs of DPO primers was designed based on ITS sequences of Leptosphaeria maculans.A DPO-PCR method was developed to detect L maculans,and the specificity and sensitivity of the method were tested.The result showed that DPO-PCR method insensitivity to annealing temperature and have a higher specificity than conventional PCR.A total 5 positive samples for Leptosphaeria maculans were detected from 10 samples by the DPO-PCR method,which was in accordance with the result by conventional PCR.DPO-PCR provided a new method to detect Leptosphaeria maculans. |
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| Keywords: | Leptosphaeria maculans DPO-PCR detection |
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