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四株异养硝化细菌的鉴定及硝化能力的初步研究
引用本文:王李宝,万夕和,徐献明,丁楠,陈颉,吴兴兵.四株异养硝化细菌的鉴定及硝化能力的初步研究[J].水产养殖,2006,27(2):7-10.
作者姓名:王李宝  万夕和  徐献明  丁楠  陈颉  吴兴兵
作者单位:1. 江苏省海洋水产研究所,江苏,南通,226007
2. 扬州大学生物科学与技术学院,江苏,扬州,225009
3. 南京师范大学生命科学学院,江苏,南京,210097
基金项目:江苏省海洋与渔业局资助项目(T9915)
摘    要:从江苏通州、海安和吕四地区的养殖池塘浅层底泥中分离得到G、H、3-4和5-5四株具明显硝化活性的异养硝化细菌,通过形态学和生理生化研究,将上述菌株初步鉴定为芽孢杆菌(Bacillus sp.)。在外加有机碳源(丙酮酸钠0.01mol/L)的条件下对各菌株进行连续培养10 d后发现,随着各供试菌株生长量的增加,培养基中铵态氮和COD的含量均有明显下降。与对照组相比,铵态氮含量最大降幅达到了8%,COD最大降幅达到了32%。

关 键 词:异养硝化细菌  硝化作用  丙酮酸钠  铵态氮  COD
文章编号:1004-2091(2006)02-0007-03
收稿时间:2005-12-26
修稿时间:2005-12-26

Primary study on the identification and nitrificationoffour heterotrophic nitrifiers fromaquacultural water
Wang Libao,Wan Xihe,Xu Xianming,Ding Nan,Chen Jie,Wu Xingbing.Primary study on the identification and nitrificationoffour heterotrophic nitrifiers fromaquacultural water[J].Journal of Aquaculture,2006,27(2):7-10.
Authors:Wang Libao  Wan Xihe  Xu Xianming  Ding Nan  Chen Jie  Wu Xingbing
Institution:1.Jiangsu Institute of Marine Fisheries, Nantong 226007, China; 2.College of Bioscience and Biotechnology, Yangzhou University Yangzhou 225009,China; 3.School of Life Science,Nanjing Normal University,Nanjing 210097,China
Abstract:The four heterotrophic nitrifierssigned C, H, 3-4 and 5-5 came from different areas of Jiangsu province. Combining the primary study on morphology and the results of physiological and biochemical tests, above-mentioned four strains were primarily indentified as Bacillus sp.. These four strains were cultivated in culture medium with sodium pyruvic injected(0.01mol/L). During the different cultural period, the concentration of ammonium-N and COD were determined. With the growth of above-mentioned tested strains, the concentration of ammonium-N and COD declined obviously.
Keywords:heterotrophic nitrifiers  nitrification  sodium pynivic  ammonium-N  COD
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