| 溶藻弧菌脂多糖对大菱鲆免疫保护实验 |
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| 引用本文： | 张伟,叶海斌,朱安成,李天保.溶藻弧菌脂多糖对大菱鲆免疫保护实验[J].水产养殖,2010,31(9):18-19. |
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| 作者姓名： | 张伟 叶海斌 朱安成 李天保 |
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| 作者单位： | 山东省海水养殖研究所,山东省海水养殖病害防治重点实验室,山东,青岛,266002 |
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| 摘 要： | 采用粗提脂多糖(LPS)对体重为110~150g大菱鲆进行免疫保护实验。注射浓度为20mg/mL和40mg/mL的LPS溶液0.2mL,以及按5‰比例添加到饲料中进行投喂试验。免疫后用0.1mL浓度为1×109CFU/mL的溶藻胶弧菌分别进行5次感染实验。结果显示:第7天即可产生免疫保护作用,RPS可达75%,免疫保护作用可持续3个月。
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| 关 键 词： | 溶藻弧菌 细菌脂多糖 大菱鲆 免疫保护率 |
Vibrio alginolyticus lipopolysaccharide on turbot immune protection test |
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| Zhang Wei,Ye Haibin,Zhu Ancheng,Li Tianbao.Vibrio alginolyticus lipopolysaccharide on turbot immune protection test[J].Journal of Aquaculture,2010,31(9):18-19. |
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| Authors: | Zhang Wei Ye Haibin Zhu Ancheng Li Tianbao |
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| Institution: | (Shandong Mariculture Research Institute, Disease Control in Shandong Province Key Laboratory of Mariculture, Qingdao 266002,Chin) |
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| Abstract: | Using crude lipopolysaccharide（LPS）on body weight of 110～150 g turbot for protective immunization. Injection concentration 20 mg/mL and 40 mg/mL of solution 0.2 mL LPS,and the ratio by 5 ‰ to the feed for feeding test. 0.1 mL after immunization with the concentration of 1×109CFU/mL of Vibrio alginolyticus gum infection experiments were carried out 5 times. The results showed that,7 days to generate immune protection,RPS 75%,and immune protection sustainable for 3 months. |
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| Keywords: | Vibrio alginolyticus Lipopolysaccharides turbot immunological protection rate |
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