| 鱼腥藻藻蓝蛋白的提取 |
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| 引用本文： | 邹宁,孙东红,焦爱霞,衣建龙.鱼腥藻藻蓝蛋白的提取[J].水产养殖,2005,26(1):5-7. |
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| 作者姓名： | 邹宁 孙东红 焦爱霞 衣建龙 |
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| 作者单位： | 1. 烟台师范学院生命科学学院,山东,烟台,264025
2. 烟台教育学院,山东,烟台,264001 |
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| 基金项目： | 教育部留学基金资助项目(415-000431) |
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| 摘 要： | 对鱼腥藻藻蓝蛋白的大规模提取方法进行了初步的探索。对实验室精细的小规模分离提取方法进行了简化。用0．01mol／L磷酸盐缓冲液浸提，经3次反复冰融渗透震荡处理，使细胞破碎，释放藻蓝蛋白，然后离心分离。用磷酸缓冲液反复提取至上清液透明。采用这种方法从鱼腥藻中提取藻蓝蛋白，可处理的样品规模大，藻蓝蛋白得率高达98．8％，并且操作方法简便，节约药品，成本低。
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| 关 键 词： | 藻蓝蛋白 提取方法 鱼腥藻 磷酸盐缓冲液 冷冻渗透震荡 |
| 文章编号： | 1004-2091(2005)01-0005-03 |
| 修稿时间： | 2004年9月14日 |
Extraction of phycocyanin from fresh- water blue- green alga Anabaena sp |
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| Zou Ning,Sun Donghong,Jiao Aixia,Yi Jianlong.Extraction of phycocyanin from fresh- water blue- green alga Anabaena sp[J].Journal of Aquaculture,2005,26(1):5-7. |
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| Authors: | Zou Ning Sun Donghong Jiao Aixia Yi Jianlong |
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| Institution: | Zou Ning1,Sun Donghong1,Jiao Aixia1,Yi Jianlong2 |
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| Abstract: | The extraction method of phycocyanin from fresh-water blue-green alga Anabaena sp. was primarily studied. A simple and efficient method was developed in this work. The cell wall of the samples was broken by freezing and thawing repeatedly (3 times) in the 0.01 mol/l phosphate buffer. Then, the phycocyanin was repeatedly (5 times) extracted from the broken cyanobacteria cells into the same buffer by centrifugation. The method is beneficial for industry production on large scale. |
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| Keywords: | Phycocyanin extraction method Anabaena sp microalga pigment extraction |
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