木瓜蛋白酶在制取斑鰶鱼蛋白中的应用

为确定木瓜蛋白酶酶解斑鰶鱼的条件。研究了酶解温度、酶浓度、酶解时间、加水量、pH值对斑鰶鱼氨基氮含量和总氮得率的影响。结果表明：温度小于55℃。随着酶解温度的提高、酶浓度的增加、酶解时间的延长斑鰶鱼酶解液氨基氮含量和总氮得率均增加；pH值对水解效果及氮回收率影响不大。综合考虑，最佳水解条件为：温度55℃。加酶量6．0‰。酶解时间1．5h．原料：水=1：2．5，pH值6．8。此时。氨基氮生成率达26．7％。总氮得率2．28％。

Study on The Technology Condition of Papain Hydrolysis Clupanodon Punctatus

In order to determine the enzymolysis conditions,this paper deals with the effects of hydrolysis temperature,enzyme concentration,hydrolysis duration,water and pH of papain on content of amino-N and T-N proportion from Clupanodon Punctatus.The results are as follows: With the increase of hydrolysis temperature,enzyme concentration and hydrolysis duration,T-N proportion and the content of amino-N raise.T-N proportion increases with the increases of water and pH has no little effect on hydrolysis and T-N proportion.In a word,the suitable hydrolysis condition are given as hydrolysis temperature of 55 ℃,enzyme concentration of 0.6 g/100g Clupanodon Punctatus,hydrolysis duration 1.5 h,proportion between Clupanodon Punctatus and water 1∶2.5 and pH7.0.The substrate concentration need not to heat.The content of amino-N is 26.1% and the T-N proportion is 2.28%.