| 甲鱼爆发性传染病病原体分离及抗血清制备 |
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| 引用本文： | 史秋梅,汤生玲.甲鱼爆发性传染病病原体分离及抗血清制备[J].河北渔业,1997(6):6-9. |
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| 作者姓名： | 史秋梅 汤生玲 |
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| 作者单位： | 河北农技师院动科系
(史秋梅，汤生玲，高桂生，马增军)，河北农技师院动科系(田在滋) |
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| 摘 要： | 从患病甲鱼的肝脏中分离到9株细菌,其中7株为温和气单胞菌,2株为豚鼠气单胞菌。两种菌JA—1和JA—2对鲫鱼的半数致死量(LD_(50))各为4.3×10~5和5.5×10~7,在两种菌培养的上清液中均有一种具有溶血活性的毒素。研究表明:甲鱼的爆发性传染病是由温和气单胞菌和豚鼠气单胞菌引起,用两种菌为菌种做成菌苗,结合免疫增效剂免疫家兔制得血清,效价可达1:512,治愈率达100％。
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| 关 键 词： | 甲鱼 气单胞菌 血清 |
ISOLATION OF PATHOGEN OF AN FULMINATING INFECTIOUS DISEASE OF SOFT-SHELLED TURTLE AND PRODUCTION OF THE ANTI-SERUM |
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| Shi Qiumei Tang Shengling Gao Guisheng,Ma Zengjun Tian Zaizi.ISOLATION OF PATHOGEN OF AN FULMINATING INFECTIOUS DISEASE OF SOFT-SHELLED TURTLE AND PRODUCTION OF THE ANTI-SERUM[J].Hebei Fisheries,1997(6):6-9. |
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| Authors: | Shi Qiumei Tang Shengling Gao Guisheng Ma Zengjun Tian Zaizi |
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| Abstract: | Nine strains of bacteria were isolated from the liver of the infected soft-shelled turtle. Among them, seven were Aeromonas sobria, the other two were Aeromonas caviac. The LD50 of the two strains, JA-1 and JA-2 to crucian carp were 4.3 ×105 and 5.5×107 respectively. There was a kind of hemolytic toxin in the supernatant of the two strains culture solution. The result showed that the fulminating infectious disease of soft-shelled turtle was caused by A. sobria and A. cariac,the titre of serum from rabbit immunited with the vaccine made from the two strains comban-ing with the immunity synergist could reach 1:512,and the cure rate was 100%. |
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| Keywords: | soft-shelled turtle Aeromonas serum |
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