三苯基锡和三丁基锡对海胆的急性毒性研究

采用发育生物学和生态毒理学的模式生物海胆检测两种典型的有机锡化合物三苯基锡(TPT)和三丁基锡(TBT)对水生生物的急性毒性,同时对上述物质对海胆的安全性进行初步评估,以期为有机锡的防控提供基础数据。半静水条件下,通过概率单位法(Bliss)得到TPT和TBT对黄海胆24h,48h,72h,96h的LC50,分别为:25.007、18.743、9.514、6.662μg·L-1和19.850、15.986、7.888、4.916μg·L-1,并计算TPT和TBT对海胆的安全浓度分别为0.666和0.492μg·L-1。结果发现,TPT和TBT对黄海胆均表现为剧毒作用,TBT毒性大于TPT。海胆对上述毒物具有很高的敏感性,结果稳定,重现性好,建议将海胆作为海洋环境中有机锡污染的监测生物。

Acute toxicity of Tributyltin Chloride and Triphenyltin Chloride on Sea urchin

We detected the acute toxicity of two typical organotin compound tributyltin chloride （TBT） and triphenyltin chloride （TPT ） on aquatic organisms by using Glyptocidaris crenularis ,the model organism for developmental biology and ecotoxicology under semi -hydrostatic conditions ,and as-sessed the security of organotin at the same time ,in order to provide the basic data for the prevention and control of organotin .Results showed that the LC50 of 24 h ,48 h ,72 h and 96 h of TPT and TBT to Glyptocidaris crenularis were 25 .007、18 .743、9 .514、6 .662 μg？L -1 and 19 .850、15 .986、7 .888、4.916 μg？L -1 ,respectively .Their safe concentration were 0 .666 ,0 .492 μg？L -1 ,respectively .Both TPT and TBT were highly toxic to Glyptocidaris crenularis ,the TBT toxicity were bigger than TPT .The sea urchin had a very high sensitivity of these poisons ,the results were stable ,with good reproducibility .We suggest sea urchin as the model organism to monitor organotin contamination in the marine environment .