碳源对海水反硝化细菌活性的影响及动力学分析

分别以乙酸钠(1.8∶1)、柠檬酸钠(3.5∶1)、葡萄糖(3∶1、6∶1、7∶1)、甲醇(3.2∶1)为唯一碳源,研究不同碳源及碳氮比对反硝化细菌活性的影响。结果表明,不同碳源及碳氮比情况下均能实现对硝酸盐的去除,其中,以乙酸钠为碳源时去除效率最高,10h去除率可达78.8%;以甲醇为碳源时去除效率最低,24h去除率为71.9%。以柠檬酸钠和甲醇作为唯一碳源时,观察到亚硝酸盐的积累,峰值分别为16.9mg.L-1、15.3mg.L-1;以葡萄糖、乙酸钠作为唯一碳源时,亚硝酸盐氮含量较低。用单一底物Monod方程模拟不同碳源条件下NO3--N去除情况,发现模拟值与实验值吻合良好。

Dynamics analysis on effects of different carbon sources on denitrifying bacteria activity

The sodium acetate(1.8:1),sodium citrate(3.5∶1),glucose(3∶1/6∶1/7∶1),methanol(3.2∶1) were chosen as sole carbon source respectively,and the influences of different kinds of carbon source and carbon nitrogen ratio on denitrifying bacteria activities were investigated.The results showed that system can achieved nitrate nitrogen removal under all conditions.Among them,when sodium acetate was chosen as sole carbon source,the highest removal efficacy was achieved by 78.8%.however,when methanol was chosen as sole carbon source,the lowest efficacy was achieved by 71.9%.Nitrite nitrogen accumulation was observed when sodium citrate and methanol were used as the sole carbon source,and peak concentration were 16.9 mg·L-1 and 15.3 mg·L-1 respectively.When glucose and sodium acetate were used as sole carbon source respectively,nitrite nitrogen accumulation didn't exist Monod equation was used to simulate the dynamics of NO3—N degradation under different carbon source conditions,and the simulated values agreed well with the experimental values.