| 镉胁迫对凡纳滨对虾血细胞基因表达的影响 |
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| 引用本文： | 张秀霞,汪蕾,张泽龙,李军涛,王冬梅,冼健安.镉胁迫对凡纳滨对虾血细胞基因表达的影响[J].河北渔业,2021(3):1-4,7. |
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| 作者姓名： | 张秀霞 汪蕾 张泽龙 李军涛 王冬梅 冼健安 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所,海南省海洋生物资源功能性成分研究与利用重点实验室,海南海口571101;中国热带农业科学院海南热带农业资源研究院,海南海口571101;华南师范大学生命科学学院,广东省水产健康安全养殖重点实验室,广州市亚热带生物多样性与环境生物监测重点实验室,广东普通高校生态与环境科学重点实验室,广东广州510631 |
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| 基金项目： | 海南省自然科学基金(319QN306);中国热带农业科学院基本科研业务费专项资金(1630052019013)。 |
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| 摘 要： | 为探讨镉(Cd)胁迫下凡纳滨对虾(Litopenaeus vannamei)血细胞的分子响应,以不同浓度的Cd2+(0,0.5 mg/L和5 mg/L)进行胁迫,于不同胁迫时间取血淋巴,测定血细胞中Trx 2、Grx 2、Grx 3和MGST 3的基因表达量变化。结果显示,Trx 2、Grx 3和MGST 3表达量在胁迫的中后期显著上调,Grx 2表达量在5 mg/L Cd2+胁迫6 h开始即有显著的升高,并持续至48 h。结果表明在Cd胁迫作用下,对虾血细胞中上调这四个氧化还原与抗氧化相关基因的表达水平,以进行胁迫防御;Grx 2对Cd胁迫较为敏感,在整个胁迫过程发挥作用,Trx 2、Grx 3和MGST 3主要在胁迫的中后期发挥作用;基因的表达响应具有一定浓度效应。
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| 关 键 词： | 凡纳滨对虾(Litopenaeus vannamei) 镉 血细胞 基因表达 |
Effects of cadmium stress on gene expression in hemocytes of the white shrimp Litopenaeus vannamei |
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| ZHANG Xiu-xia,WANG Lei,ZHANG Ze-long,LI Jun-tao,WANG Dong-mei,XIAN Jian-an.Effects of cadmium stress on gene expression in hemocytes of the white shrimp Litopenaeus vannamei[J].Hebei Fisheries,2021(3):1-4,7. |
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| Authors: | ZHANG Xiu-xia WANG Lei ZHANG Ze-long LI Jun-tao WANG Dong-mei XIAN Jian-an |
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| Institution: | (Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources,Institute of Tropical Biosciences and Biotechnology,CATAS,Haikou 571101,China;Institute of Modern Aquaculture Science and Engineering(IMASE),Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture,Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring,Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education,School of Life Sciences,South China Normal University,Guangzhou,510631,China;Hainan Institute of Tropical Agricultural Resources,CATAS,Haikou 571101,China) |
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| Abstract: | To investigate the molecular response of haemocytes of the shrimp Litopenaeus vannamei to Cd stress,gene expression levels of Trx 2,Grx 2,Grx 3 and MGST 3 were measured in haemocytes from shrimp exposed to Cd2+(0,0.5 mg/L and 5 mg/L)for different times.Results showed that the expression levels of Trx 2,Grx 3 and MGST 3 were significantly up-regulated in the middle and late stages of the stress,and the expression level of Grx 2 was significantly increased after 6 h exposure to 5 mg/L Cd2+,and this level continued to 48 h.These results indicate that expression of these four redox and antioxidant related genes in haemocytes of L.vannamei are up-regulated to protect against Cd stress.Grx 2 is sensitive to Cd stress and plays a role in the whole stress process.Trx 2,Grx 3 and MGST 3 mainly play a role in the middle and late stages of the stress.The gene expression response has a certain concentration effect. |
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| Keywords: | Litopenaeus vannamei cadmium hemocyte antioxidant enzyme gene expression |
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