| 一株海洋石油降解菌的特性分析及固定化研究 |
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| 引用本文： | 关晓燕,吴垠,毛东东,姜冰,董颖,王召会,胡超魁.一株海洋石油降解菌的特性分析及固定化研究[J].河北渔业,2016(5):1-5. |
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| 作者姓名： | 关晓燕 吴垠 毛东东 姜冰 董颖 王召会 胡超魁 |
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| 作者单位： | 1. 辽宁省海洋水产科学研究院,辽宁大连,116023;2. 大连海洋大学生命科学与技术学院,辽宁大连,116023 |
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| 基金项目： | 辽宁省海洋与渔业厅项目(201414)。 |
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| 摘 要： | 从辽东湾沿岸受石油污染的沉积物中筛选得到一株石油降解菌株BHB-16,对该菌进行了形态以及16SrDNA系统发育分析,初步确定该菌株为Lutibacterium菌属。应用沸石和珊瑚石作为载体进行该菌株的固定化研究,确定当沸石作为载体时,固定化的最佳条件为:菌株接种量为0.6mL,培养时间28h,载体投加量为10mL;选用珊瑚石为载体时,固定化的最佳条件为:菌株接种量为0.6mL,培养时间29h,载体投加量为12mL;此外,用沸石和珊瑚石固定后的菌株,其对于柴油的降解率相对于游离菌分别提高了14.4%和29.6%,初步选用珊瑚石作为载体进行菌株的固定化使其具有更好的石油降解能力。
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| 关 键 词： | 石油降解菌 固定化 沸石 珊瑚石 |
Characteristics and Immobilization of an Oil-degrading Bacteria Strain |
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| Abstract: | An oil-degrading strain BHB-16 was isolated from petroleum polluted marine sediments in Liaodong Bay ,China .It w as identified as L utibacterium genus ,according to its morphology and phylo‐genetic analysis of 16S rDNA .Then strain BHB-16 was immobilized by zeolite or corlite as the im‐mobilization carrier .The results demonstated that the optimun strain inoculation amount was 0 .6 mL , incubation time was 28 or 29 hours ,and dosage of carrier was 10 or 12 mL for zeolite or corlite ,respec‐tively .The immobilized strain presented better performance in oil degrading than free strain ,the oil degradation rate of immobilized strain was 14 .4% and 29 .6% higher than that of free strain for zeolite or corlite immobilization ,respectively . |
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| Keywords: | oil-degrading strain immobilization zeolite corlite |
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