The changes in the biochemical compositions and enzymatic activities of rotifer (<Emphasis Type="Italic">Brachionus plicatilis</Emphasis>, Müller) and <Emphasis Type="Italic">Artemia</Emphasis> during the enrichment and starvation periods |
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Authors: | Mehmet Naz |
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Institution: | (1) Faculty of Fisheries Sciences, Mustafa Kemal University, Hatay, 31040, Turkey |
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Abstract: | The changes in the biochemical compositions and enzymatic activities of rotifer (Brachionus plicatilis) and Artemia, enriched and stored at 4°C temperature, were determined. The total starvation period was 16 h and samples were taken at
the end of the 8th and 16th hours. In present study, the rotifer and nauplii catabolized a large proportion of the protein
during the enrichment period. Lipid contents of both live preys increased during the enrichment period and decreased in nauplii
and metanauplii throughout the starvation period but lipid content of the rotifer remained relatively constant during the
starvation period. The changes observed in the amino acid compositions of Artemia and the rotifer were statistically significant (P < 0.05). The conspicuous decline the essential amino acid (EAA) and nonessential amino acid (NEAA) content of the rotifer
was observed during the enrichment period. However, the essential amino acid (EAA) and nonessential amino acid (NEAA) contents
of Artemia nauplii increased during the enrichment period. The unenriched and enriched rotifers contained more monounsaturated fatty
acid (MUFAs) than polyunsaturated fatty acid (PUFAs) and saturated fatty acids (SFA). However, Artemia contained more PUFAs than MUFAs and SFA during the experimental period. A sharp increase in the amounts of docosahexaenoic
acid (DHA) during the enrichment of the rotifer and Artemia nauplii was observed. However, the amount of DHA throughout the starvation period decreased in Artemia metanauplii but not in Artemia nauplii. Significant differences in tryptic, leucine aminopeptidase N (LAP), and alkaline phosphatase (AP) enzyme activities
of Artemia and rotifer were observed during the enrichment and starvation period (P < 0.05). The digestive enzymes derived from live food to fish larvae provided the highest contribution at the end of the
enrichment period. In conclusion, the results of the study provide important contributions to determine the most suitable
live food offering time for marine fish larvae. Rotifer should be offered to fish larvae at the end of the enrichment period,
Artemia nauplii just after hatching and before being stored at 4°C, and Artemia metanauplii at the end of the enrichment and throughout the starvation period. |
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Keywords: | Artemia Amino acids Brachionus plicatilis Digestive enzymes Enrichment period Fatty acids Live food Starvation period |
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