Changes in free amino acid profile of red snapper <Emphasis Type="Italic">Lutjanus campechanus</Emphasis>, eggs,and developing larvae |
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Authors: | R P Hastey R P Phelps D A Davis K A Cummins |
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Institution: | (1) Department of Fisheries and Allied Aquacultures, Auburn University, Auburn, AL 36849, USA;(2) Department of Animal Sciences, Auburn University, Auburn, AL 36849, USA;(3) Present address: Center for Cooperative Aquaculture Research, 33 Salmon Farm Rd., Franklin, ME 04634, USA; |
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Abstract: | The free amino acids (FAA) profile was determined for newly fertilized eggs and resultant larvae from wild-caught red snapper
Lutjanus campechanus induced to spawn with hCG. Yolk sac and oil globule volumes of eggs and larvae were monitored over time from digital photographs.
FAA profiles of the eggs and larvae were measured in picomoles (pmol) of FAA/mg of eggs by HPLC. Newly fertilized eggs had
a mean total FAA content of 21.72 ± 3.55 nmoles/egg (92.81 ± 9.71 nmoles/mg eggs). Leucine, valine, lysine, and isoleucine
were the most abundant essential FAA comprising 35.9% of the total FAA. Alanine, serine, asparagine, and glycine were the
most abundant non-essential FAA comprising 34.2% of the total FAA. At 24 h post-hatch (hph) the mean total FAA had decreased
by 81% since egg fertilization. The bulk of the FAA decrease was between the time of hatch and 12 hph. Only 8.5 ± 1.5% of
the initial concentration in fertilized eggs of isoleucine, 9.7 ± 2.5% of arginine, and 9.9 ± 2.0% of threonine remained at
12 hph. Among the non-essential FAA, alanine dropped the most by 12 hph with 4.6% of the concentration found in a recently
fertilized egg remaining, while cysteine had increased 254.7 ± 26.2%. The yolk sac volume decreased rapidly in the first 12 hph
and was further reduced 77.0 ± 2.5% from 12 to 24 hph. The oil globule depletion rate was a more linear decline from fertilized
egg to 36 hph. |
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