Gonadotropin-releasing hormone in two perciform fishes: Micropogonias furnieri and Pagrus pagrus |
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| Authors: | AD Montaner LA Miranda D Vizziano A López K Okuzawa GM Somoza |
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| Institution: | (1) Department of Animal Biology, Plant Biology and Ecology, Faculty of Marine Sciences, University of Cádiz, Polígono Río San Pedro, 11510 Puerto Real, Cádiz, Spain;(2) Institute of Marine Sciences of Andalucía (CSIC), Polígono Río San Pedro, Apdo. Oficial, 11510 Puerto Real, Cádiz, Spain |
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| Abstract: | Gonadotropin-releasing hormone (GnRH) molecular variants were characterized by gradient reverse phase high performance liquid chromatography (RP)-HPLC) from brain extracts of two perciforms with economic importance for Argentina and Uruguay. RP-HPLC fractions were tested in radioimmunoassays (RIAs) with both poly-specific and specific antisera. Both species showed the presence of the same three molecular forms, immunologically and chromatographically indistinguishable from sbGnRH, cGnRH-II and sGnRH. This study supports the hypothesis that their expression is a common pattern in perciforms. |
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| Keywords: | GnRH perciform fish radioimmunoassay |
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