Relationship between biochemical constituents of fish semen and fertility: the effect of short-term storage |
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Authors: | Andrzej Ciereszko Konrad Dabrowski |
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Institution: | (1) School of Natural Resources, The Ohio State University, 2021 Coffey Road, Columbus, Ohio 43210, USA;(2) Present address: Division of Reproductive Endocrinology and Pathophysiology, Polish Academy of Sciences, Centre for Agrotechnology and Veterinary Sciences, 10-718, Olsztyn-Kortowo 5, P.O. Box 55, Poland;(3) School of Natural Resources, The Ohio State University, Columbus, OH 43210, USA |
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Abstract: | Spermatozoa and seminal plasma obtained from rainbow trout and whitefish were analyzed in respect to their aspartate aminotransferase
(AspAT) and alkaline phosphatase activities. In particular, the experiments characterized AspAT optimum pH, optimization of
assay conditions and action of coenzyme, pyridoxal 5-phosphate (vitamin B6). The effect of short-term semen storage at 0°C on biochemical indicators and fertilization rate was examined in both species.
The concentrations of reduced and oxidized ascorbic acid in seminal plasma of both species were several folds higher than
in spermatozoa and blood plasma of fish. Highly significant correlations were found for both species between AspAT activity
(sperm or seminal plasma) and fertilization rate (% of eyed-stage or hatched embryos). For rainbow trout, highly significant
correlations were found between sperm concentration, motility and fertilization rate. These results suggest that several biochemical
indicators of seminal plasma can be used as measures of sperm quality of fish. Some common biochemical parameters for fish
and mammal's semen provide evidence for using fish sperm as a model in biomedical research. |
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Keywords: | rainbow trout whitefish spermatozoa seminal plasma aspartate aminotransferase ascorbic acid |
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