酶联免疫法快速检测水产品中硝基呋喃类代谢物、氯霉素及氟苯尼考

应用特异性强的间接酶联免疫法,同时检测4种硝基呋喃类代谢物、氯霉素(CAP)和氟苯尼考(FF),建立快速分析水产品中6种药物残留量的方法。样品用盐酸进行消化,再由乙酸乙酯提取,使用酶联免疫试剂盒进行检测。实验结果显示,6种分析物均在其线性范围内,线性系数均大于0.995。在加标浓度为0.1、0.5和1.5μg/kg的加标水平下,组织样中CAP的加标回收率为60.00%~126.26%;在加标浓度为0.5、2.0和8.0μg/kg的加标水平下,组织样中FF的加标回收率为60.83%~68.37%;在加标浓度为0.1、0.5和2.0μg/kg的加标水平下,组织样中呋喃西林代谢物(SEM)的加标回收率为65.66%~148.16%;在加标浓度为0.2、1.0和4.0μg/kg的加标水平下,组织样中呋喃它酮代谢物(AMOZ)的加标回收率为69.18%~140.50%;在加标浓度为0.2、1.0和4.0μg/kg的加标水平下,组织样中呋喃妥因代谢物(AHD)的加标回收率为62.50%~131.00%;在加标水平为0.1、0.5和2.0μg/kg的加标水平下,组织样中呋喃唑酮代谢物(AOZ)的加标回收率为83.1%~144.0%。研究结果说明6种分析物的相对标准偏差均在0~7.5%之间,本方法灵敏度高、快速简便,适用于水产品中硝基呋喃类代谢物、氯霉素和氟苯尼考的快速批量检测。

Enzyme-linked immunoassay for the rapid detection of furan,chloramphenicol and florfenicol in aquatic products

This paper introduced the application of highly specific indirect enzyme-linked immunosorbent assay for the simultaneous detection of four nitrofuran metabolites,chloramphenicol and florfenicol,which established a method to quickly analyze the residues of six substances in aquatic products.Briefly,1 g of samples was digested with hydrochloric acid,then extracted with ethyl acetate,and detected using an enzyme-linked immunosorbent kit.The experimental results showed that the residual amount of the six compounds were all in good linear range with more than 0.995.Specifically,the recovery range of chloramphenicol(CAP)in tissue samples at the concentratioins of 0.1,0.5,and 1.5μg/kg was 60.00%to 126.26%.The recovery range of florfenicol(FF)in the tissue samples at the three concentrations of 0.5,2.0,and 8.0μg/kg was 60.83%to 68.37%.Tissue samples were spiked with furancillin(SEM)at 0.1,0.5,and 2.0μg/kg.The recoveries were 65.66%to 148.16%.Tissue samples were spiked with furanodone(AMOZ)at 0.2,1.0,and 4.0μg/kg,and the recovery range was 69.18%to 140.50%.Tissue samples were spiked with furanotoin(AHD)at the concentrations of 0.2,1.0,and 4.0μg/kg with the recovery range from 62.50%to 131.00%.Tissue samples with furazolidone(AOZ)at the three concentrations of 0.1,0.5,and 2.0μg/kg showed the recoveries ranged from 83.1%to 144.0%.The relative standard deviations of the above six veterinary drug residues were between 0 and 7.5%.This method is applicable to the quick determination of the metabolites including nitrofuran,chloramphenicol,and florfenicol in aquatic products.