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ISSR标记在河蟹种质检测中的应用
引用本文:郑芳,吕秀玲,孙红英,周开亚,赵强,高伟,徐信荣.ISSR标记在河蟹种质检测中的应用[J].中国水产科学,2007,14(1):46-51.
作者姓名:郑芳  吕秀玲  孙红英  周开亚  赵强  高伟  徐信荣
作者单位:南京师范大学,生命科学学院,江苏省资源生物技术重点实验室,江苏,南京,210097
基金项目:国家自然科学基金;江苏省水产三项工程项目
摘    要:将ISSR分子标记技术应用于天然水域河蟹(Eriocheir japonica)的遗传检测,结合来自母系的线粒体DNA分子标记鉴别,并通过对部分样本的细胞色素b基因变异位点测定,对ISSR标记检测结果做进一步分析和评价。对PCR/RFLP检测发现的2个携带中华亚种单元型的湛江青年河样本与来自鸭绿江、长江、闽江和南流江样本的ISSR检测与聚类分析表明:1)来自4个水系的河蟹聚为2大支,与中国大陆绒螯蟹的2个亚种分支相对应,即E.japonica sinensis和E.japonica hepuensis;2)来自南方水系湛江青年河的2个携带中华亚种单元型个体聚在北方的中华亚种(E.j.sinenesis)分支中。进一步对这2个样本的细胞色素b基因全序列测定分析表明,它们分别来源于中华亚种的ES2和ES15支系。提示ISSR标记可以提供天然水域河蟹种质混杂状况的核DNA方面的证据。同时表明,ISSR标记可以为河蟹种质资源的遗传研究提供有用的信息。中国水产科学,2007,14(1):46-51]

关 键 词:河蟹  分子标记
文章编号:1005-8737-(2007)01-0046-06
收稿时间:2006-03-02
修稿时间:2006-06-30

Application of ISSR markers in germplasm analyzing of mitten crab
ZHENG Fang,LU Xiu-ling,SUN Hong-ying,ZHOU Kai-ya,ZHAO Qiang,GAO Wei,XU Xin-rong.Application of ISSR markers in germplasm analyzing of mitten crab[J].Journal of Fishery Sciences of China,2007,14(1):46-51.
Authors:ZHENG Fang  LU Xiu-ling  SUN Hong-ying  ZHOU Kai-ya  ZHAO Qiang  GAO Wei  XU Xin-rong
Institution:Jiangsu Key Laboratory for Bioresource Technology, College of Life Sciences, Nanjing Normal University, Nanjing Jiangsu 210097, China
Abstract:The genetic admixture occurred in the mitten crabs has been inferred from populations distributed in the northern river systems of China using mitochondrial DNA markers,but the information of those ranging in the southern rivers is poorly understood.Recently,a PCR-based DNA marker,ISSR(Inter-simple sequence repeats) was widely used to investigate genetic diversification at inter-and intraspecific level.This study focused on the application of ISSR molecular marker on the genetic diagnostic analysis of the mitten crab populations.The maternal mitochondrial DNA diagnostic marker and sequencing techniques were also used in the researches to evaluate the effectiveness of ISSR analysis.The cluster analysis based on ISSR for the samples from the Yalu River,the Yangtze River,the Minjiang River and the Nanliu River,including two individuals that were sinensis haplotype determined by using 16S rDNA PCR/RFLP diagnostic marker from 24 individuals collected from Zhanjiang Young River,showed that,1) the mitten crabs from the four river systems could be divided into two clusters,corresponding to the two subspecies clades,sinensis and hepuensis;2)the two individuals from Zhanjiang Young River clustered together into the sinensis clade.The sequencing analysis of the complete cytochrome b gene for the two individuals presents that they belong to two different sinensis haplotypes ES2 and ES15.It suggests that the evidence of genetic mixture of the mitten crab in the river system could be found by using ISSR analysis.ISSR could provide useful information for the researches of mitten crab germplasm resources.Journal of Fishery Sciences of China,2007,14(1):46-51]
Keywords:ISSR
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