| 牙鲆let-7基因的克隆与表达及其靶基因预测 |
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| 引用本文： | 施志仪,吴明林,付元帅.牙鲆let-7基因的克隆与表达及其靶基因预测[J].中国水产科学,2011,18(6):1211-1218. |
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| 作者姓名： | 施志仪 吴明林 付元帅 |
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| 作者单位： | 上海海洋大学水产与生命学院,上海,201306 |
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| 基金项目： | 国家自然科学基金，上海重点学科项目 |
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| 摘 要： | let-7是时序发育过程中的重要调控因子,在促进幼体向成体转变过程中起着极其重要的作用.为探讨let-7对牙鲆(Paralichthys olivaceus)从幼体到成体转变的变态发育过程的影响,本实验对let-7进行了克隆与表达研究,并预测了其可能的靶基因.利用普通PCR技术从牙鲆中成功鉴定出let-7及其前体序列,...
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| 关 键 词： | 牙鲆 let-7 克隆与表达 变态 甲状腺激素 靶基因 |
| 修稿时间： | 2011/11/12 0:00:00 |
Cloning, expression and target gene prediction of let-7 in Paralichthys olivaceus metamorphosis |
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| SHI Zhiyi,WU Minglin,FU Yuanshuai.Cloning, expression and target gene prediction of let-7 in Paralichthys olivaceus metamorphosis[J].Journal of Fishery Sciences of China,2011,18(6):1211-1218. |
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| Authors: | SHI Zhiyi WU Minglin FU Yuanshuai |
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| Institution: | SHI Zhiyi,WU Minglin,FU Yuanshuai College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China |
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| Abstract: | The let-7 gene is a critical regulator of developmental timing events of the larval-to-adult transition.Here,Paralichthys olivaceus let-7 was identified by a stem-loop RT-PCR.The precursor of let-7 was cloned and was proven to be highly conserved among different species.Quantitative analysis revealed that the expression levels of let-7 were generally variably enhanced during the first five metamorphic stages and peaked during the late metamorphic stages(36 dph).At the end of P.olivaceus metamorphosis(41 dph... |
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| Keywords: | Paralichthys olivaceus let-7 cloning and expression metamorphosis triiodothyronine(T3) target gene |
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