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| 利用SSR﹑EST-SSR、SNP标记对鲤食物转化率、体厚、体质量3种性状的分析 | |
| 引用本文： | 王宣朋,张晓峰,李文升,张天奇,孙效文.利用SSR﹑EST-SSR、SNP标记对鲤食物转化率、体厚、体质量3种性状的分析[J].中国水产科学,2011,18(3):565-573. |
| 作者姓名： | 王宣朋 张晓峰 李文升 张天奇 孙效文 |
| 作者单位： | 1. 中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070;上海海洋大学水产与生命学院,上海201306 2. 中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨,150070 3. 中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070;大连水产学院生命科学与技术学院,辽宁大连116023 |
| 基金项目： | 国家973计划项目，农业部公益性行业科研专项 |
| 摘 要： | 利用Windows Map Manager2.0的标记回归法对鲤(Cyprinus carpio L.)F2群体进行单标记定位分析.用91个SSR标记、33个EST标记、364个SNP标记对鲤进行全基因组扫描,结果得到与食物转化率、体厚、体质量3个性状显著相关(P＜0.05)的标记,分别为27、35、27个,其中与食物转化率相关的标记SNP0041、SNP0044,其相关性达到极显著水平(P＜0.001),贡献率分别达到15%、16%.这些标记可作为分子标记辅助育种的参考标记.将得到的与食物转化率显著相关的EST座位HLJEl4、HLJE253和与体质量显著相关的HLJE253座位在NCBI上进行BLAST比对,结果显示HLJE253与斑马鱼上编码ORF2结构蛋白的基因相关,相关程度达61%.将得到的与3种性状相关的SNP标记在NCBI上进行序列查找,通过Blast比对找到相关的基因,并对可能的功能作了注释. |
| 关 键 词： | 鲤 EST-SSR SNP 食物转化率 体厚 体质量 |
| 修稿时间： | 2011/5/9 0:00:00 |
Analysis of feed conversion ratio, bodyweight, body thickness traits of common carp (Cyprinus carpio L.) using SSR, EST-SSR, SNP markers |
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| WANG Xuanpeng,ZHANG Xiaofeng,LI Wensheng,ZHANG Tianqi,SUN Xiaowen.Analysis of feed conversion ratio, bodyweight, body thickness traits of common carp (Cyprinus carpio L.) using SSR, EST-SSR, SNP markers[J].Journal of Fishery Sciences of China,2011,18(3):565-573. | |
| Authors: | WANG Xuanpeng ZHANG Xiaofeng LI Wensheng ZHANG Tianqi SUN Xiaowen |
| Institution: | WANG Xuanpeng1,2,ZHANG Xiaofeng1,LI Wensheng1,ZHANG Tianqi1,3,SUN Xiaowen1 1.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China,2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,3.College of Aqua-life Science and Technology,Dalian Fisheries University,Dalian 1160023 |
| Abstract: | An F2 hybrid common carp gynogenetic line including 68 individuals was used as mapping pane1.Markers were used to make marker regression analysis based on pseudo-testcross strategy using Windows Map Manager2.0.Ninety-one SSR markers and 33 EST makers and 364 SNP markers were selected to analyze the ge-nomic DNA of 68 progenies derived from common carp full-sib family.Twenty-seven loci were discovered that had significant impact on feed conversion efficiency and thirty-five loci were significantly related wi... |
| Keywords: | Cyprinus carpio L EST-SSR SNP feed conversion ratio body thickness body weight |
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