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Cu↑(2+)对黄鳝肝脏保护酶SOD、CAT、GSH-PX活性的影响
引用本文:鲁双庆,刘少军,刘红玉,刘筠.Cu↑(2+)对黄鳝肝脏保护酶SOD、CAT、GSH-PX活性的影响[J].中国水产科学,2002,9(2):138-141.
作者姓名:鲁双庆  刘少军  刘红玉  刘筠
作者单位:1. 湖南师范大学,生命科学学院,湖南,长沙,410081;长沙大学,应用化学与环境保护系,湖南,长沙,410003
2. 湖南师范大学,生命科学学院,湖南,长沙,410081
3. 湖南农业大学,环境科学系,湖南,长沙,410128
基金项目:湖南省自然科学基金重点课题(99JJY1003).
摘    要:以肝脏组织保护酶SOD,CAT,GSH-PX活性为指标研究了CuSO4污染对黄鳝的损伤作用,实验测得CuSO4对黄鳝的LC50为5.5mg/L,据此设定CuSO4浓度梯度,结果表明,0.7,1.5mg/L的CuSO4处理液对黄鳝的损伤较小,3.0,4.5mg/L的CuSO4处理液对黄鳝损伤较大,保护酶SOD,CAT,GSH-PX的活性在黄鳝暴露24h时被显著抑制,在处理48h后酶活性逐渐恢复并超过对照组水平,之后又回落到低于对照组水平,其变化幅度与CuSO4质量浓度呈正相关,可以证实,SOD,CAT,GSH-PX活性变化可以反映黄鳝的伤害程度。

关 键 词:保护酶  黄鳝  肝脏  SOD  CAT  GSH-PX  硫酸铜  酶活性  生态毒理学  水体污染
文章编号:1005-8737(2002)02-0138-04
修稿时间:2001年8月6日

Effects of Cu2+ on activities of protecting enzymes SOD, CAT and GSH-PX in liver tissue of Monopterus albus
LU Shuang qing ,LIU Shao jun ,LIU Hong yu ,LIU Yun.Effects of Cu2+ on activities of protecting enzymes SOD, CAT and GSH-PX in liver tissue of Monopterus albus[J].Journal of Fishery Sciences of China,2002,9(2):138-141.
Authors:LU Shuang qing    LIU Shao jun  LIU Hong yu  LIU Yun
Institution:LU Shuang qing 1,2,LIU Shao jun 1,LIU Hong yu 3,LIU Yun 1
Abstract:The concentrations of CuSO 4 were set at 0,1.0,3.0,5.0, 7.0 and 9.0 mg/L,and the LC 50 of Cu 2+ for Monopterus albus was determined at 5.5 mg/L. According to this LC 50 ,the experiment concentrations of CuSO 4 were set up at 0,0.7,1.5,3.0 and 4. 5 mg/L and the exposure periods were set up at 24,48,72,96 and 120 h. The results show that the injury is low at 0.7 and 1.5 mg/L CuSO 4, but high at 3.0 and 4.5 mg/L. The activities of protecting enzymes SOD,CAT and GSH PX in the liver tissues of M. albus are restrained obviously during 24 h treatment from 0.7 to 4.5 mg/L CuSO 4,but recover gradually and exceed the control level slowly during 48 to 72 h, and finally get down again,lower than that of the control. The changes of the activities of SOD,CAT and GSH PX can indicate the injured degree of M. albus by CuSO 4.
Keywords:Monopterus albus  liver tissue  SOD  CAT  GSH  PX  CuSO  4
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