首页 | 本学科首页   官方微博 | 高级检索  
     检索      

重组tHsp70对罗非鱼腹腔巨噬细胞免疫功能的影响
引用本文:陈明,王秋华,王瑞,甘西,李莉萍,雷爱莹,梁万文,黄维义.重组tHsp70对罗非鱼腹腔巨噬细胞免疫功能的影响[J].中国水产科学,2012,19(1):145-153.
作者姓名:陈明  王秋华  王瑞  甘西  李莉萍  雷爱莹  梁万文  黄维义
作者单位:1. 广西水产研究所,广西水产遗传育种与健康养殖重点实验室,广西南宁30021;广西大学动物科学技术学院,广西南宁30005
2. 广西大学动物科学技术学院,广西南宁30005
3. 广西水产研究所,广西水产遗传育种与健康养殖重点实验室,广西南宁30021
基金项目:广西科学研究与技术开发课题(桂科攻1123006-4);罗非鱼产业技术体系岗位科学家经费项目
摘    要:利用酵母分泌表达的重组罗非鱼热休克蛋白70(heat shock protein 70 of tilapia,tHsp70),与海豚链球菌(Streptococcus iniae)抗原在体外非共价结合形成tHsp70-抗原复合物,通过对体外培养的腹腔巨噬细胞NO释放水平、吞噬活性及免疫相关基因表达进行检测,研究tHsp70对罗非鱼细胞免疫功能的影响。结果显示,tHsp70在体外能够与海豚链球菌菌体、胞外分泌物(extracellular bacteria products,ECP)结合形成tHsp70-抗原复合物;tHsp70-抗原复合物和tHsp70均可显著增强罗非鱼腹腔巨噬细胞的贴壁和生长、NO释放及吞噬活性(P<0.01),而ECP单独作用可引起腹腔巨噬细胞死亡,显著降低腹腔巨噬细胞NO释放(P<0.05),但tHsp70与ECP体外非共价结合后可减轻ECP对巨噬细胞的损伤;tHsp70-抗原复合物和tHsp70作用于腹腔巨噬细胞,白细胞介素8(IL-8),热休克蛋白70(HSP70),CXC趋化因子受体4(CXCR4)和颗粒蛋白前体(PGRN)4个基因的表达量均显著高于海豚链球菌抗原单独刺激和无刺激对照组(P<0.01)。研究表明tHsp70具有免疫增强和免疫佐剂的功能,为开发罗非鱼链球菌病Hsp70-肽疫苗提供了实验依据。

关 键 词:热休克蛋白70  巨噬细胞  免疫功能  海豚链球菌  疫苗
修稿时间:2012/2/2 0:00:00

Effects of recombinant tHsp70 on immune function of tilapia peritoneal macrophages
CHEN Ming,WANG Qiuhu,WANG Rui,GAN Xi,LI Liping,LEI Aiying,LIANG Wanwen,HUANG Weiyi.Effects of recombinant tHsp70 on immune function of tilapia peritoneal macrophages[J].Journal of Fishery Sciences of China,2012,19(1):145-153.
Authors:CHEN Ming  WANG Qiuhu  WANG Rui  GAN Xi  LI Liping  LEI Aiying  LIANG Wanwen  HUANG Weiyi
Institution:1.Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture of Guangxi,Guangxi Institute of Fisheries,Nanning,Guangxi 530021,China; 2.Institute of Animal Science and Technology,Guangxi University,Nanning,Guangxi 530005,China
Abstract:We used recombinant tilapia Hsp70(tHsp70) from Pichia yeast to prepare non-covalent HSP70-antigen complexes with thallus and extracellular bacterial products(ECP) of Streptococcus iniae.We investigated the ef-fects of the antigen complexes on the cellular immune response of tilapia by detection of NO release,phagocytic activity,and related immune gene expression of peritoneal macrophages in vitro.tHsp70 can form complexes with the thallus and ECP of tilapia in vitro.In addition,tHsp70 alone and its complexes significantly stimulated growth and attachment of peritoneal macrophages and enhanced NO release and phagocytic activity of peritoneal macro-phages.In contrast,treatment with ECP significantly inhibited NO release and led to cell death,while treatment with tHsp70-ECP complex reduced the cell death.Moreover,tHsp70 alone or tHsp70-antigen complexes signifi-cantly increased the expression of immune related genes(IL-8,Hsp70,CXCR4,and PGRN) in tilapia peritoneal macrophages compared to antigen only and medium control groups.Our results suggest that tHsp70 has an im-munoadjuvant and immunopotentiator function,and provides experimental data for development of a tHsp70 based vaccine against tilapia streptococcal diseases.
Keywords:heat shock protein 70  macrophage  immune function  Streptococcus iniae  vaccine
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《中国水产科学》浏览原始摘要信息
点击此处可从《中国水产科学》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号