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四引物扩增受阻突变体系PCR技术在中国明对虾SNP基因分型中的研究
引用本文:张建勇,王清印,王伟继,孟宪红,孔杰,张全启.四引物扩增受阻突变体系PCR技术在中国明对虾SNP基因分型中的研究[J].中国水产科学,2011,18(4):751-759.
作者姓名:张建勇  王清印  王伟继  孟宪红  孔杰  张全启
作者单位:1. 中国海洋大学海洋生命学院,山东青岛 266002;中国水产科学研究院黄海水产研究所,山东青岛 266071;山东理工大学生命科学学院,山东淄博 255049
2. 中国水产科学研究院黄海水产研究所,山东青岛,266071
3. 中国海洋大学海洋生命学院,山东青岛,266002
基金项目:国家自然科学基金资助项目,山东省自然科学基金项目资助
摘    要:采用四引物扩增受阻突变体系PCR(Tetra-primer ARMS-PCR)技术,对中国明对虾(Fenneropenaeus chinensis)的80个单核苷酸多态性位点(Single Nucleotide Polymorphisms,SNPs)进行验证.结果表明,通过优化PCR反应条件、调整内外引物浓度和采取To...

关 键 词:单核苷酸多态性  基因分型  中国明对虾  四引物扩增受阻突变体系PCR
修稿时间:2011/8/19 0:00:00

tetra-primer amplification refractory mutation system-polymerase chain reaction in SNP genotyping of shrimp Fenneropenaeus chinensis
ZHANG Jianyong,WANG Qingyin,WANG Weiji,MENG Xianhong,KONG Jie,ZHANG Quanqi.tetra-primer amplification refractory mutation system-polymerase chain reaction in SNP genotyping of shrimp Fenneropenaeus chinensis[J].Journal of Fishery Sciences of China,2011,18(4):751-759.
Authors:ZHANG Jianyong  WANG Qingyin  WANG Weiji  MENG Xianhong  KONG Jie  ZHANG Quanqi
Institution:ZHANG Jianyong1,2,3,WANG Qingyin2,WANG Weiji2,MENG Xianhong2,KONG Jie2,ZHANG Quanqi1 1.College of Marine Life Sciences,Ocean University of China,Qingdao 266002,China,2.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,3.School of Life Sciences,Shandong University of Technology,Zibo 255049
Abstract:Tetra-primer amplification refractory mutation system-polymerase chain reaction(Tetra-primer ARMA-PCR) was introduced to investigate single nucleotide polymorphisms(SNPs) genotyping in Chinese shrimp(Fenneropenaeus chinensis) and 80 putative SNPs loci were studied.Twenty out of the 80 SNP tetra-primer ARMA-PCR primer sets were validated following touchdown profiles and the outer and the expected inner bands were amplified.Homozygous and heterozygous were detected by agarose gel and the genotypes were obtain...
Keywords:single nucleotide polymorphisms(SNP)  genotyping  Fenneropenaeus chinensis  tetra-primer ARMA-PCR  
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