| 运动强度对斜带石斑鱼生长、非特异性免疫和肝脏抗氧化能力的影响 |
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| 引用本文： | 魏小岚,虞顺年,阳艳,肖雅元,刘永,韦芳三,唐文乔,李纯厚.运动强度对斜带石斑鱼生长、非特异性免疫和肝脏抗氧化能力的影响[J].中国水产科学,2017,24(5):1055-1064. |
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| 作者姓名： | 魏小岚 虞顺年 阳艳 肖雅元 刘永 韦芳三 唐文乔 李纯厚 |
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| 作者单位： | 1. 中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东省渔业生态环境重点实验室,广东广州510300;上海海洋大学水产与生命学院,上海201306;贵州省农业科学院水产研究院,贵州贵阳550025;2. 中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东省渔业生态环境重点实验室,广东广州510300;上海海洋大学水产与生命学院,上海201306;3. 贵州省农业科学院水产研究院,贵州贵阳,550025;4. 中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东省渔业生态环境重点实验室,广东广州510300;5. 上海海洋大学水产与生命学院,上海,201306 |
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| 基金项目： | 公益性行业(农业)科研专项项目(201403008)，农业部南海渔业资源开发利用重点实验室开放基金课题(FREU2015-03)，中央级公益性科研院所基本科研业务费专项项目(2015161) |
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| 摘 要： | 为探讨有氧运动强度对斜带石斑鱼(Epinephelus coioides)生长、血液非特异性免疫机能和肝脏抗氧化能力的影响,本研究将斜带石斑鱼放置在4个运动强度(对照组0 bl/s、实验组0.5 bl/s、实验组1.0 bl/s和实验组2.0 bl/s)中进行8周的训练实验。结果表明,1.0 bl/s运动强度对斜带石斑鱼的特定生长率(SGR)、增重率(WGR)和成活率(SR)有显著提高作用(P0.05)。血清中总蛋白(TP)、球蛋白(GLB)、补体C3含量和溶菌酶(LYZ)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)活性随运动强度的增加先上升后下降,其中1.0 bl/s组显著高于其他组(P0.05),而血清谷丙转氨酶(GPT)和谷草转氨酶(GOT)活性先下降后上升,其中1.0 bl/s组显著低于其他组(P0.05),白蛋白(ALB)和补体C4含量无显著性差异(P0.05)。肝脏总抗氧化能力(T-AOC)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)的活性随运动强度的增加也呈现先增加后降低的趋势,在1.0 bl/s组中活性显著高于其他组(P0.05)。8周运动训练对肝脏HSP70mRNA表达水平有显著影响,1.0 bl/s组显著高于其他组(P0.05)。总之,1.0 bl/s运动强度可提高斜带石斑鱼幼鱼的生长速度、增强血液非特异性免疫功能和肝脏抗氧化能力,上调HSP70 mRNA表达水平。
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| 关 键 词： | 运动训练 成活率 非特异性免疫功能 特定生长率 |
| 修稿时间： | 2017/9/12 0:00:00 |
Effects of exercise intensity on growth,blood innate immunity,hepatic antioxidant capacity,and HSPs70 mRNA expression of Epinephelus coioides |
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| WEI Xiaolan,YU Shunnian,YANG Yan,XIAO Yayuan,LIU Yong,WEI Fangsan,TANG Wenqiao,LI Chunhou.Effects of exercise intensity on growth,blood innate immunity,hepatic antioxidant capacity,and HSPs70 mRNA expression of Epinephelus coioides[J].Journal of Fishery Sciences of China,2017,24(5):1055-1064. |
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| Authors: | WEI Xiaolan YU Shunnian YANG Yan XIAO Yayuan LIU Yong WEI Fangsan TANG Wenqiao LI Chunhou |
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| Institution: | 1. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture;Guangdong Provincial Key Laboratory of Fishery Ecology Environment;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China;2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;3. Fisheries Research Institute, Guizhou Academy of Agricultural Science, Guiyang 550025, China |
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| Abstract: | The present study was conducted to investigate the effects of exercise training on the growth,serum innate immunity,and hepatic antioxidant capacity of orange-spotted grouper (Epinephelus coioides).The fish were exercised at an intensity of 0,0.5,1,and 2 body lengths per second (bl/s) for 8 weeks.Specific growth,weight gain,and survival increased significantly in the 1 bl/s group (P＜0.05).The plasma concentrations of total protein,globulin protein,complement 3,lysozyme,alkaline-phosphatase,and acid phosphatase increased as exercise intensity increased up to 1 bl/s and then decreased at the higher intensity of 2 bl/s (P＜0.05).The plasma contents of glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase decreased as the exercise intensity increased up to 1 bl/s,and then increased at the higher intensity of 2 bl/s (P＜0.05).The albumin and complement 4 contents of plasma did not significantly differ among treatments.Hepatic total antioxidant capacity,catalase,and superoxide dismutase activity increased with exercise intensity up to 1 bl/s and then decreased at 2 bl/s (P＜0.05).The exercise treatments significantly affected the mRNA expression of HSPs70,and that of the 1 bl/s group was the highest.The results indicated that an exercise intensity of 1 bl/s effectively enhanced the growth,serum innate immunity,hepatic antioxidant capacity,and HSPs70 mRNA expression of Epinephelus coioides. |
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| Keywords: | exercise training survival rate innate immunity specific growth rate |
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