首页 | 本学科首页   官方微博 | 高级检索  
     检索      

4 个斧文蛤群体微卫星标记的遗传多样性分析
引用本文:滕爽爽,方军,金文辉,肖国强,邵艳卿,柴雪良,张炯明.4 个斧文蛤群体微卫星标记的遗传多样性分析[J].中国水产科学,2015,22(3):580-587.
作者姓名:滕爽爽  方军  金文辉  肖国强  邵艳卿  柴雪良  张炯明
作者单位:浙江省海洋水产养殖研究所;浙江省近岸水域生物资源开发和保护重点实验室;温州医科大学
基金项目:海洋公益性行业科研专项子任务(201205021-5);国家贝类产业技术体系项目(CARS-48)
摘    要:为研究斧文蛤(Meretrix lamarckii)不同地理群体(浙江苍南群体、福建长乐群体、福建宁德群体以及广东汕头群体)的遗传结构和系统发生关系,采用13对微卫星分子标记,对4个不同地理群体进行了分析。结果显示,13对引物共检测出63个等位基因,每个位点等位基因数(Na)2~7个,平均每个位点观测等位基因数(Na)为4.87;有效等位基因数(Ne)为1.927~2.591;平均观测杂合度(Ho)为0.437~0.562;平均期望杂合度(He)为0.446~0.549;平均多态信息含量(PIC)为0.383~0.490;Hardy-Weinberg平衡检验表明,4个群体的大部分微卫星位点都偏离平衡状态(P0.05);UPMGA聚类分析表明,浙江苍南群体与福建宁德群体聚为一支,福建长乐群体和广东汕头群体聚为一支;群体间遗传变异指数Fst为0.230 9,表明4个斧文蛤群体间的遗传变异为23.09%。研究表明,斧文蛤4个不同地理群体遗传多样性处于中等多态水平;4个群体的遗传距离与它们实际的地理分布情况基本一致;4个地理群体间的变异较大,遗传分化水平较高。该研究为斧文蛤种质资源的有效保存、管理和恢复提供了理论依据。

关 键 词:斧文蛤  微卫星  遗传多样性
修稿时间:2015/5/12 0:00:00

Analysis of genetic diversity in four different Meretrix lamarckii geo- graphical populations using microsatellite markers
TENG Shuangshuang,FANG Jun,JIN Wenhui,XIAO Guoqiang,SHAO Yanqing,CHAI Xueliang,ZHANG Jiongming.Analysis of genetic diversity in four different Meretrix lamarckii geo- graphical populations using microsatellite markers[J].Journal of Fishery Sciences of China,2015,22(3):580-587.
Authors:TENG Shuangshuang  FANG Jun  JIN Wenhui  XIAO Guoqiang  SHAO Yanqing  CHAI Xueliang  ZHANG Jiongming
Institution:TENG Shuangshuang;FANG Jun;JIN Wenhui;XIAO Guoqiang;SHAO Yanqing;CHAI Xueliang;ZHANG Jiongming;Zhejiang Mariculture Research Institute;Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource;Wenzhou Medical University;
Abstract:

 Thirteen polymorphic microsatellite markers were examined in four different Meretrix lamarckii geographi- cal populations to assess the naturally occurring Meretrix lamarckii resource. The four populations were sampled in Cangnan City, Zhejiang Province; Changle City, Fujian Province; Ningde City, Fujian Province; and Shantou City, Guangdong Province. Sixty-three alleles were found in the 13 microsatellite markers. Allele number per locus was 2–7, and the mean number of alleles was 4.87. Mean effective number of alleles, mean observed and expected heterozygosity, and mean polymorphism information content were 1.927–2.591, 0.437–0.562, 0.446–0.549, and 0.383–0.490, respec- tively. All of these genetic indices indicate medium genetic diversity in the four populations. The Hardy-Weinberg bal- ance test showed that most of the microsatellite sites in the four populations deviated from equilibrium(P<0.05). An unweighted pair group method with arithmetic mean cluster analysis indicated that the Cangnan population clustered with the Ningde population into one clade, and the Changle and Shantou populations were in a second group, which was consistent with their actual geographical locations. The Fst value was 0.230 9, suggesting that the genetic variation in the four M. lamarckii populations was 23.09%, which was high, and that the genetic differentiation level was high. 

Keywords:Meretrix lamarckii  microsatellite  genetic diversity
本文献已被 CNKI 等数据库收录!
点击此处可从《中国水产科学》浏览原始摘要信息
点击此处可从《中国水产科学》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号